Synthesis of steroid tracers with a oxyme ligation method and use thereof in fluorescent polarisation immunoassay
- Autores: Prokhorenko I.A.1, Glushchenko D.A.1, Gulyak E.L.1, Mikhura I.V.1, Korshun V.A.1, Mukhametova L.I.2, Eremin S.А.2
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Afiliações:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
- Lomonosov Moscow State University
- Edição: Volume 50, Nº 1 (2024)
- Páginas: 51-64
- Seção: Articles
- URL: https://archivog.com/0132-3423/article/view/671002
- DOI: https://doi.org/10.31857/S0132342324010055
- EDN: https://elibrary.ru/OWPZNS
- ID: 671002
Citar
Resumo
A method for the synthesis of ketosteroid oxime conjugates with a fluorescein (6-FAM) derivative connected via a short bifunctional aminooxypropyl linker is proposed. The conjugates were used as tracers for fluorescence polarization immunoassay (FPIA) with monoclonal antibodies. Stereoisomers by the oxime group were separated by preparative RP TLC and HPLC and tested in the FPIA method. Binding parameters of of tracers with monoclonal antibodies (mAbs) to progesterone were studied. Tracers showed high efficiency in the analysis of steroid hormones, the detection limit of progesterone being lower than that for a previously described analog. The Z-isomer was found to be more sensitive in the FPIA analysis compared to the E-isomer.
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Sobre autores
I. Prokhorenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Autor responsável pela correspondência
Email: prig67@mail.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997
D. Glushchenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: prig67@mail.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997
E. Gulyak
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: prig67@mail.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997
I. Mikhura
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: prig67@mail.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997
V. Korshun
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: prig67@mail.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997
L. Mukhametova
Lomonosov Moscow State University
Email: prig67@mail.ru
Department of Chemistry
Rússia, Leninskiye Gory 1/3, Moscow, 119991S. Eremin
Lomonosov Moscow State University
Email: prig67@mail.ru
Department of Chemistry
Rússia, Leninskiye Gory 1/3, Moscow, 119991Bibliografia
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