The use of fluorescence time-resolved microscopy to increase the endoplasmic reticulum selectivity of arylidene-imidazolones fluorogenic dyes
- Authors: Gilvanov A.R.1, Smirnov A.Y.1, Krasnova S.A.1, Solovyev I.D.2, Savitsky A.P.2, Bogdanova Y.A.1, Baranov M.S.1,3
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
- A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
- Pirogov Russian National Research Medical University
- Issue: Vol 50, No 5 (2024)
- Pages: 694-701
- Section: ПИСЬМА РЕДАКТОРУ
- URL: https://archivog.com/0132-3423/article/view/670817
- DOI: https://doi.org/10.31857/S0132342324050129
- EDN: https://elibrary.ru/LQOADH
- ID: 670817
Cite item
Abstract
Using fluorescence time-resolved microscopy (FLIM), a number of previously synthesized fluorogenic aryliden-imidazolone analogues, predominantly staining the endoplasmic reticulum (ER) of living cells, were studied. It has been shown that the use of this type of fluorescence microscopy can increase the selectivity of ER staining.
Keywords
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About the authors
A. R. Gilvanov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Author for correspondence.
Email: aidar_gilvanov@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
A. Y. Smirnov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: aidar_gilvanov@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
S. A. Krasnova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: aidar_gilvanov@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
I. D. Solovyev
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
Email: aidar_gilvanov@mail.ru
Russian Federation, Leninskiy prosp. 33/2, 119071 Moscow
A. P. Savitsky
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
Email: aidar_gilvanov@mail.ru
Russian Federation, Leninskiy prosp. 33/2, 119071 Moscow
Yu. A. Bogdanova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: aidar_gilvanov@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
M. S. Baranov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University
Email: aidar_gilvanov@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997; ul. Ostrovitianova 1, Moscow, 117997
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