Marine invertebrate lectins: isolation, properties and biological activity

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Abstract

Interest in marine organisms is due to the high content of biologically active substances in them, which are objects of fundamental and applied biomedical research and are effective in the development of therapeutic and prophylactic agents against a wide range of diseases. The laboratory of chemistry of non-infectious immunity of PIBOC FEB RAS conducts research on the screening, isolation, structure determination, study of physicochemical properties and biological activity of lectins from marine invertebrates. Lectins of different carbohydrate specificity have been isolated from bivalve mollusks, the physiological role of which is to participate in the innate immunity of mollusks. These proteins have different biological activity, including antibacterial and antiproliferative.

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About the authors

Irina V. Chikalovets

Elyakov Pacific Institute of Bioorganic Chemistry of FEB RAS

Author for correspondence.
Email: ivchik6@mail.ru
ORCID iD: 0000-0001-5102-9311

Candidate of Sciences in Chemistry, Senior Researcher

Russian Federation, Vladivostok

Tatyana O. Mizgina

Elyakov Pacific Institute of Bioorganic Chemistry of FEB RAS

Email: tanya.tasha@mail.ru
ORCID iD: 0000-0002-9587-897X

Candidate of Sciences in Chemistry, Junior Researcher

Russian Federation, Vladivostok

Alina P. Filshtein

Elyakov Pacific Institute of Bioorganic Chemistry of FEB RAS

Email: alishichka@mail.ru
ORCID iD: 0009-0000-6293-6582

Candidate of Sciences in Chemistry, Junior Researcher

Russian Federation, Vladivostok

Alexandra S. Kuzmich

Elyakov Pacific Institute of Bioorganic Chemistry of FEB RAS

Email: assavina@mail.ru
ORCID iD: 0000-0001-5358-5880

Researcher

Russian Federation, Vladivostok

Oleg V. Chernikov

Elyakov Pacific Institute of Bioorganic Chemistry of FEB RAS

Email: chernikov@piboc.dvo.ru
ORCID iD: 0000-0002-3076-3637

Candidate of Sciences in Biology, Deputy Director

Russian Federation, Vladivostok

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Supplementary files

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2. Fig. 1. Antiproliferative effect of lectins against cell lines of different types of human intestinal and breast tumours as determined by MTS test. Data are presented as mean value ± standard deviation (n = 3)

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3. Fig. 2. Effect of lectins on tumour cell adhesion. Cells were incubated in the presence (100 µg/ml) or absence (control) of lectins. The results were detected using a light microscope

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4. Fig. 3. Effect of CGL (A) and MTL (B) on the cell cycle of HT-29 cells

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5. Fig. 4. Effect of lectins on AFC production in HT-29 and MCF-7 tumour cells. Data are presented as mean value ± standard deviation (n = 3)

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6. Fig. 5. Effect of lectins on AFC production in Raji tumour cells

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7. Fig. 6. Effect of lectins on the proliferation of HT-29, MCF-7 and Raji tumour cells in the presence of NAC

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8. Fig. 7. Effect of CGL lectin on autophagy in Raji cells of Burkitt's lymphoma cells

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9. Fig. 8. Effect of CGL (A, B) and MTL (B) on tumour cells of HT-29 and MCF-7 lines determined by soft agar method

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10. Fig. 9. Effect of CGL on migration of HT-29, Raji and MCF-7 tumour cell lines

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11. Fig. 10. Effect of CGL on migration of HT-29 tumour cell line by the scratch overgrowth method

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