Isolation, physico-chemical characteristics and acute toxicity determination of water-soluble polysaccharide from basidial raw material Ganoderma lucidum

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As a result of the study, branched polysaccharides were isolated from the basidiomycete raw materials of Ganoderma lucidum. It has been established that the isolated fractions contain branched polysaccharides in the form of complexes with melanin. After purification of polysaccharides by ion-exchange chromatography, two fractions were obtained from basidial raw materials: neutral polysaccharides GW-1 with a yield of 25.71% and anionic polysaccharides GW-2, the yield of which was 5.26%, respectively. The physicochemical properties of the obtained samples were studied by IR and UV spectroscopy. The degree of purity of the obtained fractions of branched polysaccharides was established. Using gas chromatography, one-dimensional (13C NMR, 1H NMR) and two-dimensional (COSY, TOCSY, HSQC, HMBC, NOESY) NMR spectroscopy, the composition and molecular structure of the obtained polysaccharide samples were determined. The results showed that the isolated and purified polysaccharides are branched glucans with 1,4,6- and 1,3,6-bonds between glucopyranose units. Pharmacotoxicological studies were carried out on white outbred mice and it was found that the resulting polysaccharides belong to class V, practically non-toxic compounds (LD50 ≥ 2000 mg/kg). Isolated polysaccharides (GW) are promising biologically active components, on the basis of which it is possible to create drugs with hepatoprotective and antitumor activity.

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Sobre autores

S. Khaytmetova

Institute of Bioorganic Chemistry named A.S. Sadykov AS RUz

Autor responsável pela correspondência
Email: xsb75@mail.ru
Uzbequistão, ul. Mirzo Ulugbek 83, Tashkent, 100125

A. Turaev

Institute of Bioorganic Chemistry named A.S. Sadykov AS RUz

Email: xsb75@mail.ru
Uzbequistão, ul. Mirzo Ulugbek 83, Tashkent, 100125

G. Khalilova

Institute of Bioorganic Chemistry named A.S. Sadykov AS RUz

Email: xsb75@mail.ru
Uzbequistão, ul. Mirzo Ulugbek 83, Tashkent, 100125

B. Muhitdinov

Institute of Bioorganic Chemistry named A.S. Sadykov AS RUz

Email: xsb75@mail.ru
Uzbequistão, ul. Mirzo Ulugbek 83, Tashkent, 100125

Bibliografia

  1. Wasser P., Weis L. // Int. J. Med. Mushrooms. 1999. V. 1. Р. 31–62. https://doi.org/10.1615/IntJMedMushrooms.v1.i1.30
  2. Wasser S.P. // Appl. Microbiol. Biotechnol. 2002. V. 60. P. 258–274. https://doi.org/10.1007/s00253-002-1076-7
  3. Mizuno T. // Foods Food Ingred. J. Jpn. 1996. V. 167. Р. 69–85.
  4. Mizuno T. // Food Rev. Int. 1995. V. 11. Р. 173–178.
  5. Gorin P.A.J., Barreto-Berger E. // The Chemistry of Polysaccharides of Fungi and Lichens. In: The Polysaccharides / Ed. Aspinall G.O. New York: Academic Press, 1983. Р. 365–409.
  6. Liu W., Lu W., Chai Y., Liu Y., Yao W., Gao Х. // Carbohydr. Polym. 2017. V. 176. P. 140–151. https://doi.org/10.1016/j.carbpol.2017.08.071
  7. Guo Q., Ai L., Cui S.W. // Strategies for Structural Characterization of Polysaccharides. In: Methodology for Structural Analysis of Polysaccharides. SpringerBriefs in Molecular Science. Springer, Cham, 2018. P. 20–25.
  8. Pan D., Wang L., Chen C., Teng B., Wang Ch., Xu Z., Hu B., Zhou P. // Food Chem. 2012. V. 135. Р.1097–1103.
  9. Magdeldin S., Moser A.C. // Affinity Chromatography: Principles and Applications / Ed. Magdeldin S. InTech, 2012. P. 3–28. http://dx.doi.org/10.5772/39087
  10. Wang Q., Zhao X., Pu J., Luan X. // Carbohydr. Polym. 2016. V. 143. P. 296–300. https://doi.org/10.1016/j.carbpol.2016.02.023
  11. Kim Y., Kim B., Cheong Ch., David L., Williams, Kim Ch., Lim S. // Carbohydr. Res. 2000. V. 328. P. 331–341. https://doi.org/ 10.1016/s0008-6215(00)00105-1
  12. Patel B.K., Campanella O.H., Janaswamy S. // Carbohydr. Polym. 2013. V. 92. P. 1873–1879. https://doi.org/10.1016/j.carbpol.2012.11.026
  13. Tao Y., Zhang R., Wei Y., Liu H., Yang H., Zhao Q. // Carbohydr. Polym. 2015. V. 128. P. 179–187. https://doi.org/10.1016/j.carbpol.2015.04.012
  14. Сушинская Н.В., Кукулянская Т.А, Курченко В.П., Шостак Л.М. // Химия и технология органических веществ. 2004. № 4. С. 193-196.
  15. Халилова Г.А., Мухитдинов Б.И., Хайтметова С.Б., Нормахаматов Н.С., Тураев А.С. // Узбекский химический журнал 2019. № 2 с. 48-55.
  16. Arun G., Eyini M., Gunasekaran P. // J. Exp. Biol. 2015. V. 53. Р. 380–387.
  17. Wang Y., Liu Y., Yu H., Zhou S., Zhang Z., Wu Di., Yan M., Tang Q., Zhang J. // Carbohydr. Polym. 2017. V. 167. Р. 337–344. https://doi.org/10.1016/j.carbpol.2017.03.016
  18. Ding H.H., Cui S.W., Goff H.D, Chen J., Guo Q., Wang Q. // Carbohydr. Polym. 2016. V. 151. P. 538–545. https://doi.org/10.1016/j.carbpol.2016.05.094
  19. Ren Y., Bai Y., Zhang Z., Cai W., Del R., Flores A. // Molecules. 2019. V. 24. Р. 3122. https://doi.org/10.3390/molecules24173122
  20. Халилова Г.А., Тураев А.С., Мухитдинов Б.И., Хайтметова С.Б., Азимова Л.Б., Нормахаматов Н.С. // Докл. Акад. наук Респ. Узбекистан. 2020. № 5. C. 55–62.
  21. Manna D.K., Nandi A.K., Pattanayak M., Maity P., Tripathy S., Mandal A.K., Roy S., Tripathy S.S., Gupta N., Islam S.S. // Carbohydr. Polym. 2015. V. 134. P. 375–384. https://doi.org/10.1016/j.carbpol.2015.07.099
  22. Yang B., Prasad K.N., Jiang Y. // Carbohydr. Polym. 2016. V. 137. P. 570–575. https://doi.org/10.1016/j.carbpol.2015.10.088
  23. Халилова Г.А., Тураев А.С., Мухитдинов Б.И., Хайтметова С.Б., Нормахаматов Н.С. // Хим.-фарм. журн. 2022. Т. 56. С. 21–24. https://doi.org/10.30906/0023-1134-2022-56-8-21-24
  24. Хайтметова С.Б., Тураев А.С., Халилова Г.А., Тагайалиева Н.А., Аббосхонова М.О. // Эксп. клин. фарм. 2022. Т. 85. № 12. С. 38–41. https://doi.org/10.30906/0869-2092-2022-85-12-38-41.
  25. Сидиров К.К. // Токсикология новых промышленных химических веществ. Классификация веществ по токсичности / М.: Медицина, 1973. Вып. 3. C. 47.
  26. ГОСТ 32644–2014. Методы испытания по воздействию химической продукции на организм человека. Острая пероральная токсичность – метод определения класса острой токсичности (OECD, Test № 423:2001, IDT). М.: Стандартинформ, 2015.
  27. BeMiller J.N. // Food Anal. 2017. Chapter 19. P. 333–360. https://doi.org/10.1007/978-3-319-45776-5_19.
  28. Masuko T., Minami A., Iwasaki N., Majima T., Nishimura S.I., Lee Y.C. // Anal. Biochem. 2004. V. 339. P. 69–72. https://doi.org/ 10.1016/j.ab.2004.12.001
  29. Khaytmetova S.B., Turaeva A.S., Khalilova G.A. // Polymer Sci. Ser. B. 2022. V. 64. Р. 500–505. https://doi.org/10.1134/S1560090422700221
  30. Björndal H., Hellerqvist C. G., Lindberg B., Svensson S. // Ang. Chem. Int. Ed. English. 1970. V. 9. Р. 610–619. https://doi.org/10.1002/anie.197006101
  31. Арзамасцев Е.В., Березовская И.В., Верстакова О.Л., Гуськова Т.А., Дурнев А.Д., Иванова А.С., Крепкова Л.В., Сорокина А.В. // Методические рекомендации по изучению общетоксического действия лекарственных средств / Руководство по проведению доклинических исследований лекарственных средств. Часть первая. М.: Гриф и К, 2012. С. 13–24.
  32. Руководство по экспериментальному (доклиническому) изучению новых фармакологических веществ / Под общей ред. чл.-корр. РАМН, проф. Р.У. Хабриева (2 изд., перераб. и доп.). М.: Медицина, 2005. 832 с.
  33. OECD Guideline for Testing of Chemicals. Acute Oral Toxicity – Fixed Dose Procedure. 2001. № 420. https://ntp.niehs.nih.gov/sites/default/files/iccvam/suppdocs/feddocs/oecd/oecd_gl420.pdf

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2. Fig. 1. Structure of β-glucans of basidiomycetes.

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3. Fig. 2. UV spectrum of water-soluble fractions of polysaccharides isolated from basidial raw materials of Ganoderma lucidum.

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4. Fig. 3. Chromatogram of the separation of polysaccharides GW-1 and GW-2 on an ion exchange column with DEAE-52-cellulose (eluent 0–1 M NaCl gradient, elution rate 1 ml/min).

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5. Fig. 4. IR spectra of samples of crude (GW) and purified (GW-1 and GW2) polysaccharide fractions from Ganoderma lucidum.

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6. Fig. 5. Permethylated alditol acetals: 1,5-di-O-acetyl-2,3,4,6-tetra-O-methyl-glucitol (I); 1,3,5-tri-O-acetyl-2,4,6-tri-O-methyl-glucitol (II); 1,4,5-tri-O-acetyl-2,3,6-tri-O-methyl-glucitol (III); 1,5,6-tri-O-acetyl-2,3,4-tri-O-methylglucitol (IV); 1,4,5,6-tetra-O-acetyl-2,3-di-O-methylglucitol (V); 1,3,5,6-tetra-O-acetyl-2,4-di-O-methyl-glucitol (VI).

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7. Fig. 6. Spectra of 13C-NMR (a), 1H-NMR (b), HSQC (c) and HMBC (d) of the GW-1 polysaccharide.

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8. Fig. 7. Binding order, monosaccharide sequence and possible polysaccharide structures of GW-1.

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