Antimicrobial metabolites from pig nasal microbiota

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The mammal microbiome is considered an attractive source of bioactive compounds, including antibiotics. In this work, we studied cultivable microorganisms from the nasal microbiota of the Hungarian domestic pig (Sus domesticus). Taxonomy positions of the 20 isolated strains (18 bacteria, 1 yeast, 1 fungus) were determined by phylogenetic analysis, morphological study and a substrate utilization assay. The strains were subjected to antibiotic susceptibility testing and antimicrobial activity screening. Pseudomonas aeruginosa strain SM-11 was found to produce 4 known antibacterial molecules (pyocyanine, pyochelin, pyoluteorin, monorhamnolipid). Production of pyocyanine was induced by cocultivation with test microorganisms Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922. The results suggest that the mammal microbiota might serve as a valuable source of antimicrobial-producing strains, including those of rare taxa. Cocultivation techniques are promising approach to explore antimicrobials from silent biosynthetic gene clusters.

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作者简介

A. Baranova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: alferovava@gmail.com
俄罗斯联邦, 117997, Moscow, ul. Miklukho-Maklaya, 16/10

Y. Zakalyukina

Lomonosov Moscow State University

Email: alferovava@gmail.com

Department of Soil Science

俄罗斯联邦, 119991, Moscow, ul. Leninskie Gory, 1/12

A. Tyurin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: alferovava@gmail.com
俄罗斯联邦, 117997, Moscow, ul. Miklukho-Maklaya, 16/10

V. Korshun

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: alferovava@gmail.com
俄罗斯联邦, 117997, Moscow, ul. Miklukho-Maklaya, 16/10

O. Belozerova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: alferovava@gmail.com
俄罗斯联邦, 117997, Moscow, ul. Miklukho-Maklaya, 16/10

M. Biryukov

Lomonosov Moscow State University

Email: alferovava@gmail.com

Department of Biology

俄罗斯联邦, 119991, Moscow, ul. Leninskie Gory, 1/12

A. Moiseenko

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Lomonosov Moscow State University

Email: alferovava@gmail.com

Department of Biology

俄罗斯联邦, 117997, Moscow, ul. Miklukho-Maklaya, 16/10; 119991, Moscow, ul. Leninskie Gory, 1/12

S. Terekhov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: alferovava@gmail.com
俄罗斯联邦, 117997, Moscow, ul. Miklukho-Maklaya, 16/10

V. Alferova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: alferovava@gmail.com
俄罗斯联邦, 117997, Moscow, ul. Miklukho-Maklaya, 16/10

参考

  1. Hutchings M.I., Truman A.W., Wilkinson B. // Curr. Opin. Microbiol. 2019. V. 51. P. 72–80. https://doi.org/10.1016/j.mib.2019.10.008
  2. Miethke M., Pieroni M., Weber T., Brönstrup M., Hammann P., Halby L., Arimondo P.B., Glaser P., Aigle B., Bode H.B., Moreira R., Li Y., Luzhetskyy A., Medema M. H., Pernodet J., Stadler M., Tormo J.R., Genilloud O., Truman A.W., Weissman K.J., Takano E., Sabatini S., Stegmann E., Brötz-Oesterhelt H., Wohlleben W., Seemann M., Empting M., Hirsch A.K.H., Loretz B., Lehr C.M., Titz A., Herrmann J., Jaeger T., Alt S., Hesterkamp T., Winterhalter M., Schiefer A., Pfarr K., Hoerauf A., Graz H., Graz M., Lindvall M., Ramurthy S., Karlén A., Dongen M., Petkovic H., Keller A., Peyrane F., Donadio S., Fraisse L., Piddock L.J.V., Gilbert I.H., Moser H.E, Müller R. // Nat. Rev. Chem. 2021. V. 5. P. 726–749. https://doi.org/10.1038/s41570-021-00313-1
  3. Bernal F.A., Hammann P., Kloss F. // Curr. Opin. Biotechnol. 2022. V. 78. P. 102783. https://doi.org/10.1016/j.copbio.2022.102783
  4. Cook M.A., Wright G.D. // Sci. Transl. Med. 2022. V. 14. P. eabo7793. https://doi.org/10.1126/scitranslmed.abo7793
  5. Dai J., Han R., Xu Y., Li N., Wang J., Dan W. // Bioorg. Chem. 2020. V. 101. P. 103922. https://doi.org/10.1016/j.bioorg.2020.103922
  6. Atanasov A., Zotchev S., Dirsch V., Orhan I., Banach M., Rollinger J., Barreca D., Weckwerth W., Bauer R., Edward B., Majeed M., Bishayee A., Bochkov V., Bonn G., Braidy N., Bucar F., Cifuentes A., D’Onofrio G., Bodkin M., Supuran C. // Nat. Rev. Drug Discov. 2021. V. 20. P. 1–17. https://doi.org/10.1038/s41573-020-00114-z
  7. Newman D.J., Cragg G.M. // J. Nat. Prod. 2020. V. 83. P. 770–803. https://doi.org/10.1021/acs.jnatprod.9b01285
  8. Baranova A.A., Alferova V.A., Korshun V.A., Tyurin A.P. // Life. 2023. V. 13. P. 1073. https://doi.org/10.3390/life13051073
  9. Walesch S., Birkelbach J., Jézéquel G., Haeckl F.P.J., Hegemann J.D., Hesterkamp T., Hirsch A.K.H., Hammann P., Müller R. // EMBO Rep. 2023. V. 24. P. e56033. https://doi.org/10.15252/embr.202256033
  10. Баранова А.А., Алферова В.А., Коршун В.А., Тюрин А.П. // Биоорг. химия. 2020. Т. 46. С. 593–665. [Baranova A.A., Alferova V.A., Korshun V.A., Tyurin A.P. // Russ. J. Bioorg. Chem. 2020. V. 46. P. 903–971.] https://doi.org/10.1134/S1068162020060023
  11. Baranova A.A., Zakalyukina Y.V., Ovcharenko A.A., Korshun V.A., Tyurin A.P. // Biology (Basel). 2022. V. 11. P. 1676. https://doi.org/10.3390/biology11111676
  12. Abdelaleem E.R., Samy M.N., Abdelmohsen U.R., Desoukey S.Y. // Lett. Appl. Microbiol. 2022. V. 74. P. 8–16. https://doi.org/10.1111/lam.13559
  13. Imai Y., Meyer K.J., Iinishi A., Favre-Godal Q., Green R., Manuse S., Caboni M., Mori M., Niles S., Ghiglieri M., Honrao C., Ma X., Guo J.J., Makriyannis A., Linares-Otoya L., Böhringer N., Wuisan Z.G., Kaur H., Wu R., Mateus A., Typas A., Savitski M.M., Espinoza J.L., O’Rourke A., Nelson K.E., Hiller S., Noinaj N., Schäberle T.F., D’Onofrio A., Lewis K. // Nature. 2019. V. 576. P. 459–464. https://doi.org/10.1038/s41586-019-1791-1
  14. Wang L., Ravichandran V., Yin Y., Yin J., Zhang Y. // Trends Biotechnol. 2019. V. 37. P. 492–504. https://doi.org/10.1016/j.tibtech.2018.10.003
  15. Donia M.S., Fischbach M.A. // Science. 2015. V. 349. P. 1254766. https://doi.org/10.1126/science.1254766
  16. Mousa W.K., Athar B., Merwin N.J., Magarvey N.A. // Nat. Prod. Rep. 2017. V. 34. P. 1302–1331. https://doi.org/10.1039/C7NP00021A
  17. Chiumento S., Roblin C., Kieffer-Jaquinod S., Tachon S., Leprètre C., Basset C., Aditiyarini D., Olleik H., Nicoletti C., Bornet O., Iranzo O., Maresca M., Hardré R., Fons M., Giardina T., Devillard E., Guerlesquin F., Couté Y., Atta M., Perrier J., Lafond M., Duarte V. // Sci. Adv. 2019. V. 5. P. eaaw9969. https://doi.org/10.1126/sciadv.aaw9969
  18. Barber C.C., Zhang W. // J. Ind. Microbiol. Biotechnol. 2021. V. 48. P. kuab010. https://doi.org/10.1093/jimb/kuab010
  19. Lewis K. // Cell. 2020. V. 181. P. 29–45. https://doi.org/10.1016/j.cell.2020.02.056
  20. Pirolo M., Espinosa-Gongora C., Alberdi A., Eisenho- fer R., Soverini M., Eriksen E.Ø., Pedersen K.S., Guardabassi L. // Anim. Microbiome. 2023. V. 5. P. 5. https://doi.org/10.1186/s42523-023-00226-y
  21. Petrelli S., Buglione M., Rivieccio E., Ricca E., Baccigalupi L., Scala G., Fulgione D. // Anim. Microbiome. 2023. V. 5. P. 14. https://doi.org/10.1186/s42523-023-00235-x
  22. Vasco K., Guevara N., Mosquera J., Zapata S., Zhang L. // Anim. Microbiome. 2022. V. 4. P. 65. https://doi.org/10.1186/s42523-022-00218-4
  23. Kauter A., Epping L., Semmler T., Antao E.-M., Kannapin D., Stoeckle S.D., Gehlen H., Lübke-Becker A., Günther S., Wieler L.H., Walther B. // Anim. Microbiome. 2019. V. 1. P. 14. https://doi.org/10.1186/s42523-019-0013-3
  24. O’Sullivan J.N., Rea M.C., O’Connor P.M., Hill C., Ross R.P. // FEMS Microbiol. Ecol. 2019. V. 95. P. fiy241. https://doi.org/10.1093/femsec/fiy241
  25. Wertz P.W., De Szalay S. // Antibiotics. 2020. V. 9. P. 159. https://doi.org/10.3390/antibiotics9040159
  26. O’Sullivan J.N., O’Connor P.M., Rea M.C., O’Sulli- van O., Walsh C.J., Healy B., Mathur H., Field D., Hill C., Ross R.P. // J. Bacteriol. 2020. V. 202. P. e00639-19. https://doi.org/10.1128/JB.00639-19
  27. O’Neill A.M., Worthing K.A., Kulkarni N., Li F., Nakatsuji T., McGrosso D., Mills R.H., Kalla G., Cheng J.Y., Norris J.M., Pogliano K., Pogliano J., Gonzalez D.J., Gallo R.L. // eLife. 2021. V. 10. P. e66793. https://doi.org/10.7554/eLife.66793
  28. Swaney M.H., Kalan L.R. // Infect. Immun. 2021. V. 89. P. e00695-20. https://doi.org/10.1128/IAI.00695-20
  29. Heilbronner S., Krismer B., Brötz-Oesterhelt H., Peschel A. // Nat. Rev. Microbiol. 2021. V. 19. P. 726–739. https://doi.org/10.1038/s41579-021-00569-w
  30. Terekhov S.S., Smirnov I.V., Malakhova M.V., Samoi- lov A.E., Manolov A.I., Nazarov A.S., Danilov D.V., Dubiley S.A., Osterman I.A., Rubtsova M.P., Kostryukova E.S., Ziganshin R.H., Kornienko M.A., Vanyushkina A.A., Bukato O.N., Ilina E.N., Vlasov V.V., Severinov K.V., Gabibov A.G., Altman S. // PNAS. 2018. V. 115. P. 9551–9556. https://doi.org/10.1073/pnas.1811250115
  31. Covington B.C., Seyedsayamdost M.R. // J. Am. Chem. Soc. 2022. V. 144. P. 14997–15001. https://doi.org/10.1021/jacs.2c05790
  32. Egerszegi I., Rátky J., Solti L., Brüssow K.-P. // Arch. Anim. Breed. 2003. V. 46. P. 245–256. https://doi.org/10.5194/aab-46-245-2003
  33. Breed cards: Mangalitsa (Swallow-Belly Manga- litsa) Pig. https://www.pig333.com/articles/breed-cards-mangalitsa-swallow-belly-mangalitsa-pig_15977/
  34. Alhede M., Qvortrup K., Liebrechts R., Høiby N., Givskov M., Bjarnsholt T. // FEMS Immunol. Med. Microbiol. 2012. V. 65. P. 335–342. https://doi.org/10.1111/j.1574-695X.2012.00956.x
  35. Tihlaříková E., Neděla V., Đorđević B. // Sci. Rep. 2019. V. 9. P. 2300. https://doi.org/10.1038/s41598-019-38835-w
  36. Muscariello L., Rosso F., Marino G., Giordano A., Barbarisi M., Cafiero G., Barbarisi A. // J. Cell. Physiol. 2005. V. 205. P. 328–334. https://doi.org/10.1002/jcp.20444
  37. Bergmans L., Moisiadis P., Van Meerbeek B., Quirynen M., Lambrechts P. // Int. Endod. J. 2005. V. 38. P. 775–788. https://doi.org/10.1111/j.1365-2591.2005.00999.x
  38. Grund E., Kroppenstedt R.M. // Int. J. Syst. Evol. Microbiol. 1990. V. 40. P. 5–11. https://doi.org/10.1099/00207713-40-1-5
  39. Wei Q., Ma L. // Int. J. Mol. Sci. 2013. V. 14. P. 20983– 21005. https://doi.org/10.3390/ijms141020983
  40. Brandel J., Humbert N., Elhabiri M., Schalk I.J., Mislin G.L.A., Albrecht-Gary A.-M. // Dalton Trans. 2012. V. 41. P. 2820. https://doi.org/10.1039/c1dt11804h
  41. Abdelaziz A.A., Kamer A.M.A., Al-Monofy K.B., Al-Madboly L.A. // Microb. Cell Fact. 2022. V. 21. P. 262. https://doi.org/10.1186/s12934-022-01988-x
  42. Brodhagen M., Henkels M.D., Loper J.E. // Appl. Environ. Microbiol. 2004. V. 70. P. 1758–1766. https://doi.org/10.1128/AEM.70.3.1758-1766.2004
  43. Esposito R., Speciale I., De Castro C., D’Errico G., Russo Krauss I. // Int. J. Mol. Sci. 2023. V. 24. P. 5395. https://doi.org/10.3390/ijms24065395
  44. Gogineni V., Chen X., Hanna G., Mayasari D., Hamann M.T. // J. Antibiot. (Tokyo). 2020. V. 73. P. 490–503. https://doi.org/10.1038/s41429-020-0321-6
  45. Masson F., Lemaitre B. // Microbiol. Mol. Biol. Rev. 2020. V. 84. P. e00089-20. https://doi.org/10.1128/MMBR.00089-20
  46. Olofsson T.C., Butler È., Markowicz P., Lindholm C., Larsson L., Vásquez A. // Int. Wound J. 2016. V. 13. P. 668–679. https://doi.org/10.1111/iwj.12345
  47. Varijakzhan D., Loh J.-Y., Yap W.-S., Yusoff K., Seboussi R., Lim S.-H.E., Lai K.-S., Chong C.-M. // Marine Drugs. 2021. V. 19. P. 246. https://doi.org/10.3390/md19050246
  48. Abd-Elgawad M.M.M. // Life. 2022. V. 12. P. 1360. https://doi.org/10.3390/life12091360
  49. Bassols A., Costa C., Eckersall P.D., Osada J., Sabrià J., Tibau J. // Proteomics Clin. Appl. 2014. V. 8. P. 715–731. https://doi.org/10.1002/prca.201300099
  50. Heinritz S.N., Mosenthin R., Weiss E. // Nutr. Res. Rev. 2013. V. 26. P. 191–209. https://doi.org/10.1017/S0954422413000152
  51. Espinosa-Gongora C., Larsen N., Schønning K., Fredholm M., Guardabassi L. // PLoS One. 2016. V. 11. P. e0160331. https://doi.org/10.1371/journal.pone.0160331
  52. Chlebicz A., Śliżewska K. // Int. J. Environ. Res. Public Health. 2018. V. 15. P. 863. https://doi.org/10.3390/ijerph15050863
  53. Meurens F., Summerfield A., Nauwynck H., Saif L., Ger- dts V. // Trends Microbiol. 2012. V. 20. P. 50–57. https://doi.org/10.1016/j.tim.2011.11.002
  54. Gaskins H.R. // In: Swine Nutrition / Eds. Lewis A.J, Southern L.L. CRC Press, 2000. P. 585–609. https://doi.org/10.1201/9781420041842
  55. Crespo-Piazuelo D., Estellé J., Revilla M., Criado-Mesas L., Ramayo-Caldas Y., Óvilo C., Fernández A.I., Ballester M., Folch J.M. // Sci. Rep. 2018. V. 8. P. 12727. https://doi.org/10.1038/s41598-018-30932-6
  56. Isaacson R., Kim H.B. // Anim. Health Res. Rev. 2012. V. 13. P. 100–109. https://doi.org/10.1017/S1466252312000084
  57. Correa-Fiz F., Gonçalves dos Santos J.M., Illas F., Aragon V. // Sci. Rep. 2019. V. 9. P. 6545. https://doi.org/10.1038/s41598-019-43022-y
  58. Correa-Fiz F., Fraile L., Aragon V. // BMC Genomics. 2016. V. 17. P. 404. https://doi.org/10.1186/s12864-016-2700-8
  59. Obregon-Gutierrez P., Aragon V., Correa-Fiz F. // Pathogens. 2021. V. 10. P. 697. https://doi.org/10.3390/pathogens10060697
  60. Correa-Fiz F., Neila-Ibáñez C., López-Soria S., Napp S., Martinez B., Sobrevia L., Tibble S., Aragon V., Migura-Garcia L. // Sci. Rep. 2020. V. 10. P. 20354. https://doi.org/10.1038/s41598-020-77313-6
  61. Wang T., He Q., Yao W., Shao Y., Li J., Huang F. // Front. Microbiol. 2019. V. 10. P. 1083. https://doi.org/10.3389/fmicb.2019.01083
  62. Dai H., Chen A., Wang Y., Lu B., Wang Y., Chen J., Huang Y., Li Z., Fang Y., Xiao T., Cai H., Du Z., Wei Q., Kan B., Wang D. // Int. J. Syst. Evol. Microbiol. 2019. V. 69. P. 852–858. https://doi.org/10.1099/ijsem.0.003248
  63. Matias Rodrigues J.F., Schmidt T.S.B., Tackmann J., Mering C. von // Bioinformatics. 2017. V. 33. P. 3808– 3810. https://doi.org/10.1093/bioinformatics/btx517
  64. Wang F., Gai Y., Chen M., Xiao X. // Int. J. Syst. Evol. Microbiol. 2009. V. 59. P. 2759–2762. https://doi.org/10.1099/ijs.0.008912-0
  65. Touchette D., Altshuler I., Gostinčar C., Zalar P., Raymond-Bouchard I., Zajc J., McKay C.P., Gunde-Cimerman N., Whyte L.G. // ISME J. 2022. V. 16. P. 221–232. https://doi.org/10.1038/s41396-021-01030-9
  66. Raza M., Zhang Z.-F., Hyde K.D., Diao Y.-Z., Cai L. // Fungal Diversity. 2019. V. 99. P. 1–104. https://doi.org/10.1007/s13225-019-00434-5
  67. Bennur T., Ravi Kumar A., Zinjarde S.S., Javdekar V. // J. Appl. Microbiol. 2016. V. 120. P. 1–16. https://doi.org/10.1111/jam.12950
  68. Xu D., Nepal K.K., Chen J., Harmody D., Zhu H., McCarthy P.J., Wright A.E., Wang G. // Synth. Syst. Biotechnol. 2018. V. 3. P. 246–251. https://doi.org/10.1016/j.synbio.2018.10.008
  69. Vela A.I., Sánchez-Porro C., Aragón V., Olvera A., Domínguez L., Ventosa A., Fernández-Garayzábal J.F. // Int. J. Syst. Evol. Microbiol. 2010. V. 60. P. 2446–2450. https://doi.org/10.1099/ijs.0.016626-0
  70. Vela A.I., Arroyo E., Aragon V., Sanchez-Porro C., Latre M.V., Cerda-Cuellar M., Ventosa A., Dominguez L., Fernandez-Garayzabal J.F. // Int. J. Syst. Evol. Microbiol. 2009. V. 59. P. 671–674. https://doi.org/10.1099/ijs.0.006205-0
  71. Fusco V., Quero G.M., Cho G.-S., Kabisch J., Meske D., Neve H., Bockelmann W., Franz C.M.A.P. // Front. Microbiol. 2015. V. 6. P. 155. https://doi.org/10.3389/fmicb.2015.00155
  72. Borgo F., Ballestriero F., Ferrario C., Fortina M.G. // Ann. Microbiol. 2015. V. 65. P. 833–839. https://doi.org/10.1007/s13213-014-0924-x
  73. Gaaloul N., Ben Braiek, O., Berjeaud J.M., Arthur T., Cavera V.L., Chikindas M.L., Hani K., Ghrairi T. // J. Food Saf. 2014. V. 34. P. 300–311. https://doi.org/10.1111/jfs.12126
  74. Guarino A., Giannella R., Thompson M.R. // Infect. Immun. 1989. V. 57. P. 649–652. https://doi.org/10.1128/iai.57.2.649-652.1989
  75. Rieusset L., Rey M., Muller D., Vacheron J., Gerin F., Dubost A., Comte G., Prigent-Combaret C. // Microb. Biotechnol. 2020. V. 13. P. 1562–1580. https://doi.org/10.1111/1751-7915.13598
  76. Kudo S., Morimoto Y.V., Nakamura S. // Microbiology. 2015. V. 161. P. 701–707. https://doi.org/10.1099/mic.0.000031
  77. Dickerman A., Bandara A.B., Inzana T.J. // Int. J. Syst. Evol. Microbiol. 2020. V. 70. P. 180–186. https://doi.org/10.1099/ijsem.0.003730
  78. Fuller A.T., Horton J.M. // J. Gen. Microbiol. 1950. V. 4. P. 417–433. https://doi.org/10.1099/00221287-4-3-417
  79. Caulier S., Nannan C., Gillis A., Licciardi F., Bragard C., Mahillon J. // Front. Microbiol. 2019. V. 10. P. 302. https://doi.org/10.3389/fmicb.2019.00302
  80. Tyurin A., Alferova V., Korshun V. // Microorganisms. 2018. V. 6. P. 52. https://doi.org/10.3390/microorganisms6020052
  81. Björkroth K.J., Schillinger U., Geisen R., Weiss N., Hoste B., Holzapfel W.H., Korkeala H.J., Vandam- me P. // Int. J. Syst. Evol. Microbiol. 2002. V. 52. P. 141–148. https://doi.org/10.1099/00207713-52-1-141
  82. Fortina M.G., Ricci G., Mora D., Manachini P.L. // Int. J. Syst. Evol. Microbiol. 2004. V. 54. P. 1717–1721. https://doi.org/10.1099/ijs.0.63190-0
  83. Jančič U., Gorgieva S. // Pharmaceutics. 2021. V. 14. P. 76. https://doi.org/10.3390/pharmaceutics14010076
  84. Muthukrishnan P., Chithra Devi D., Mostafa A.A., Alsamhary K.I., Abdel-Raouf N., Nageh Sholkamy E. // J. Infect. Public Health. 2020. V. 13. P. 1522–1532. https://doi.org/10.1016/j.jiph.2020.06.025
  85. Baranova A.A., Chistov A.A., Tyurin A.P., Prokhorenko I.A., Korshun V.A., Biryukov M.V., Alferova V.A., Zakalyukina Y.V. // Microorganisms. 2020. V. 8. P. 1948. https://doi.org/10.3390/microorganisms8121948
  86. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, 11th Ed. // Clinical and Laboratory Standards Institute (CLSI): Wayne, USA, 2015. https://clsi.org/standards/products/microbiology/documents/m07/
  87. Performance Standards for Antimicrobial Susceptibility Testing: 25th Informational Supplement // Clinical and Laboratory Standards Institute (CLSI): Wayne, USA, 2015. https://clsi.org/media/1631/m02a12_sample.pdf
  88. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, 3rd Ed. // Clinical and Laboratory Standards Institute (CLSI): Wayne, USA, 2008. https://clsi.org/media/1461/m27a3_sample.pdf
  89. Smith A.C., Hussey M.A. // Gram Stain Protocols. American Society for Microbiology, 2005. https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol-2886.pdf
  90. Glass N.L., Donaldson G.C. // Appl. Environ. Microbiol. 1995. V. 61. P. 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  91. White T.J., Bruns T., Lee S., Taylor J. // In: PCR Protocols. A Guide to Methods and Applications. Academic Press, Cambridge, Massachusetts, U.S., 1990. P. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  92. Lane D.J., Stackebrandt E., Goodfellow M. // Nucleic Acid Techniques in Bacterial Systematic. Wiley, Hoboken, New Jersey, U.S., 1991.
  93. Glauert A.M. // Practical Methods in Electron Microscopy. North-Holland Publishing Company, Amsterdam, London, 1974.
  94. Wang M., Carver J.J., Phelan V.V., Sanchez L.M., Garg N., Peng Y., Nguyen D.D., Watrous J., Kapono C.A., Luzzatto-Knaan T., Porto C., Bouslimani A., Melnik A.V., Meehan M.J., Liu W.-T., Crüsemann M., Boudreau P.D., Esquenazi E., Sandoval-Calderón M., Kersten R.D., Pace L.A., Quinn R.A., Duncan K.R., Hsu C.-C., Floros D.J., Gavilan R.G., Kleigrewe K., Northen T., Dutton R.J., Parrot D., Carlson E.E., Aigle B., Michelsen C.F., Jelsbak L., Sohlenkamp C., Pevzner P., Edlund A., McLean J., Piel J., Murphy B.T., Gerwick L., Liaw C.-C., Yang Y.-L., Humpf H.-U., Maansson M., Keyzers R.A., Sims A.C., Johnson A.R., Sidebottom A.M., Sedio B.E., Klitgaard A., Larson C.B., Boya P C.A., Torres-Mendoza D., Gonzalez D.J., Silva D.B., Marques L.M., Demarque D.P., Pociute E., O’Neill E.C., Briand E., Helfrich E.J.N., Granato- sky E.A., Glukhov E., Ryffel F., Houson H., Mohima- ni H., Kharbush J.J., Zeng Y., Vorholt J.A., Kurita K.L., Charusanti P., McPhail K.L., Nielsen K.F., Vuong L., Elfeki M., Traxler M.F., Engene N., Koyama N., Vin- ing O.B., Baric R., Silva R.R., Mascuch S.J., Tomasi S., Jenkins S., Macherla V., Hoffman T., Agarwal V., Williams P.G., Dai J., Neupane R., Gurr J., Rodrí- guez A.M.C., Lamsa A., Zhang C., Dorrestein K., Duggan B.M., Almaliti J., Allard P.-M., Phapale P., Nothias L.-F., Alexandrov T., Litaudon M., Wolfen- der J.-L., Kyle J.E., Metz T.O., Peryea T., Nguyen D.-T., VanLeer D., Shinn P., Jadhav A., Müller R., Waters K.M., Shi W., Liu X., Zhang L., Knight R., Jensen P.R., Palsson B.Ø., Pogliano K., Linington R.G., Gutiérrez M., Lopes N.P., Gerwick W.H., Moore B.S., Dorrestein P.C., Bandeira N. // Nat. Biotechnol. 2016. V.34. P. 828–837. https://doi.org/10.1038/nbt.3597

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2. Fig. 1. Ubiquitous presence and constant circulation of microorganisms in ecosystems.

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3. Fig. 2. Scanning electron microscopy of the Nocardiopsis alba SM-1 strain grown on a Muller–Hinton medium for 5 days at 28°C. The arrows show rounded sporangia-like structures.

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4. Fig. 3. Morphology of Rhodotorula frigidialcoholis (SM-6): light microscopy (a), scanning electron micrography after 3 days of incubation on a Muller–Hinton medium at 28°C (b).

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5. Fig. 4. Image from a scanning electron microscope of a biofilm of the Pseudomonas aeruginosa SM-11 strain formed after 48 hours on an LB medium at 37°C.

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6. Fig. 5. Micromorphology of Chaetomium anastomosans SM-20 on PDA medium after 7 days of incubation at 25 ± 1 °C: mature fruit bodies (perithecia) examined by light microscopy (a) and scanning electron microscope (b), perithecium hairs in SEM (c), ascospores in SEM (d) and in ESEM (d, e).

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7. Fig. 6. HPLC profile (controlled by UV absorption at 350 nm) and elution conditions (blue line) for SM-11-MeCN20 (a). UV spectra and fragmentation pattern of MS2 ([M + H]+, HCD mass spectrum in positively charged ion registration mode) for pyocyanin (b) and pyohelin (c).

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8. Fig. 7. HPLC profile (controlled by UV absorption at 350 nm) and elution conditions (blue line) for SM-11-MeCN50 (a); UV spectrum and MS2 fragmentation scheme ([M – H]–, in the mode of registration of negatively charged ions) for pyolyuteorin (b).

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9. Fig. 8. HPLC profile (controlled by UV absorption at 210 nm) and elution conditions (blue line) for SM-11-MeCN 100 (a). UV spectrum and MS2 fragmentation scheme for RhaC10C10 monoramnolipid (b).

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10. Fig. 9. Collection of samples and isolation of microorganisms.

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11. Fig. 10. Design of experiments to test the antagonistic activity of isolated microorganisms: (a) – the method of transverse bands, (b) – the method of agar blocks.

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12. Additional materials
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