Obtaining of active amino groups on a surface of a polyethylene terephthalate films and their quantitative evaluation

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Abstract

A method has been developed for obtaining active amino groups on the surface of a polyethylene terephthalate (PET) substrate. A method has been developed to quantify the concentration and distribution of chemically accessible amino groups on the surface of a PET substrate using the cyanine dye Cy5 and digital fluorescence microscopy. Amino groups can be used for further chemical modification of the PET surface, grafting of various functional groups and covalent binding with biomolecules, which opens up prospects for widespread use of inexpensive PET as functional substrates in biochips, biosensors, “laboratory-on-a-chip” devices and other biotechnological applications.

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About the authors

G. F. Shtylev

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

I. Y. Shishkin

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

S. A. Lapa

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

V. E. Shershov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

V. E. Barsky

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

S. A. Polyakov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

V. A. Vasiliskov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

O. A. Zasedateleva

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

V. E. Kuznetsova

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

A. V. Chudinov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Author for correspondence.
Email: chud@eimb.ru
Russian Federation, ul. Vavilova 32, Moscow, 119991

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2. Fig. 1. Scheme of chemical modification of the PET film surface.

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3. Fig. 2. Scheme of synthesis of fluorescent dye Cy5 and activated derivative of para-nitrophenyl ether of dye Cy5-pNP.

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4. Fig. 3. Micrograph of a PET substrate in the light of Cy5 dye fluorescence after amination with a 10% aqueous solution of ethylenediamine for 8 h at 70°C and binding of amino groups to the Cy5-pNP fluorescent dye. A graph of the signal distribution along the drawn line on the fluorescence image is shown.

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