Hepatoprotective efficacy of the use of oxymethyl uracil in various experimental models
- Autores: Khusnutdinova N.Y.1, Repina E.F.1, Karimov D.O.1, Timasheva G.V.1, Yakupova T.G.1, Mustafin A.G.2, Smolyankin D.A.1
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Afiliações:
- Ufa Research Institute of Occupational Health and Human Ecology
- Ufa Institute of Chemistry, RAS
- Edição: Volume 100, Nº 11 (2021)
- Páginas: 1278-1282
- Seção: PREVENTIVE TOXICOLOGY AND HYGIENIC STANDARTIZATION
- ##submission.datePublished##: 06.12.2021
- URL: https://archivog.com/0016-9900/article/view/638776
- DOI: https://doi.org/10.47470/0016-9900-2021-100-11-1278-1282
- ID: 638776
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Resumo
Introduction. The formation of toxic liver damage is carried out with the participation of various mechanisms of pathological processes. Chemicals, drugs and alcohol play a significant role. Timely and correct selection of correction drugs will help reduce the risk of toxic organ damage.
The aim of the study is a comparative assessment of the hepatoprotective activity of oxymethyluracil in the early stages of exposure to various toxic agents.
Materials and methods. The studies were carried out on male outbred white rats with a single injection of hepatotoxicants (carbon tetrachloride, paracetamol, ethanol) and a correction drug — oxymethyl uracil — followed by the study of biochemical parameters 1 and 3 days after the introduction of the chemical agent.
Results. As a result of the studies carried out, it was found that specific metabolic changes are noted already one day after exposure to toxicants in the body of animals. Correction of tetrachloromethane and paracetamol intoxication with oxymethyl uracil leads to the normalization of the functional state of hepatocytes.
Conclusion. Oxymethyl uracil exhibits hepatoprotective properties already in the early stages of acute toxic liver damage (1-3 days), being most effective in tetrachloromethane and paracetamol intoxication.
Contribution:
Khusnutdinova N.Yu. — concept and design of the study, writing the text;
Repina E.F. — concept and design of the study, collection of material and data processing, editing;
Karimov D.O., Yakupova T.G., Smolyankin D.A. — a collection of material and data processing;
Timasheva G.V. — a collection of material and data processing, statistical processing;
Mustafin A.G. — oxymethyl uracil synthesis.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The work was carried out at the expense of subsidies to implement a state task within the framework of the sectoral research program of Rospotrebnadzor “Hygienic scientific substantiation of minimizing risks to the health of the population of Russia” for 2016-2020 on topic 3.5, State no. registration AAAA-A16-116022610045-4.
Conclusion of the bioethical commission: the study was approved by the bioethical commission of the “Ufa Research Institute of Occupational Health and Human Ecology”, carried out per the European Convention for the Protection of Vertebrate Animals Used for Experiments or Other Scientific Purposes (ETS N 123), the directive of the European Parliament and the Council of the European Union 2010/63/EU of 22.09.2010 on the protection of animals used for scientific purposes.
Received: June 02, 2021 / Accepted: September 28, 2021 / Published: November 30, 2021
Palavras-chave
Sobre autores
Nadezhda Khusnutdinova
Ufa Research Institute of Occupational Health and Human Ecology
Autor responsável pela correspondência
Email: husnutdinova.n76@gmail.com
ORCID ID: 0000-0001-5596-8180
MD, Researcher, Department of Toxicology and Genetics with the Experimental Clinic of Laboratory Animals, Ufa Research
Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation.
e-mail: husnutdinova.n76@gmail.com
RússiaElvira Repina
Ufa Research Institute of Occupational Health and Human Ecology
Email: noemail@neicon.ru
ORCID ID: 0000-0001-8798-0846
Rússia
Denis Karimov
Ufa Research Institute of Occupational Health and Human Ecology
Email: noemail@neicon.ru
ORCID ID: 0000-0003-0039-6757
Rússia
Gulnara Timasheva
Ufa Research Institute of Occupational Health and Human Ecology
Email: noemail@neicon.ru
ORCID ID: 0000-0003-2435-6936
Rússia
Tat’yana Yakupova
Ufa Research Institute of Occupational Health and Human Ecology
Email: noemail@neicon.ru
ORCID ID: 0000-0002-1236-8246
Rússia
Akhat Mustafin
Ufa Institute of Chemistry, RAS
Email: noemail@neicon.ru
ORCID ID: 0000-0002-8342-8787
Rússia
Denis Smolyankin
Ufa Research Institute of Occupational Health and Human Ecology
Email: noemail@neicon.ru
ORCID ID: 0000-0002-7957-2399
Rússia
Bibliografia
- Makhmudov K.A., Gutnikova A.R., Ablyaeva N.Kh., Bazhenov L.G., Kasymov A.Kh. Comparative evaluation of different methods of detoxification in case of pesticide intoxication of the body. Toksikologicheskiy vestnik. 2004; (2): 9–12. (in Russian)
- Kutsenko S.A. Fundamentals of Toxicology: Scientific and Methodological Edition [Osnovy toksikologii: nauchno-metodicheskoe izdanie]. St. Petersburg: Foliant; 2004. (in Russian)
- Antonenko O.M. Hepatotoxicity: options for pharmacological correction. Meditsinskiy sovet. 2013; (6): 45–51. (in Russian)
- Agzamova G.S., Alieva A.M. Clinical peculiarities and treatment of toxic hepatitis (review of literature). Meditsina truda i promyshlennaya ekologiya. 2009; (12): 44–7. (in Russian)
- Golikov S.N., Sanotskiy N.V., Tiunov L.A. General Mechanisms of Toxic Action [Obshchie mekhanizmy toksicheskogo deystviya]. Moscow; 1986. (in Russian)
- Abdullaev N.Kh., Karimov Kh.Ya. Liver in Case of Intoxication with Hepatropic Poisons [Pechen’ pri intoksikatsii gepatropnymi yadami]. Tashkent; 1989. (in Russian)
- Okovityy S.V., Bezborodkina N.N., Zarubina I.V., Mironova O.P., Kudryavtsev B.N., Shulenin S.N. Studying the hepatoprotector effect of bemithyl on a model of chronic toxic liver damage. Eksperimental’naya i klinicheskaya farmakologiya. 2006; (2): 52–4. (in Russian)
- Zarezade V., Moludi J., Mostafazadeh M., Mohammadi M., Veisi A. Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl4-induced hepatotoxicity in rats. Avicenna J. Phytomed. 2018; 8(1): 51–62.
- Rudakov V.S., Voskanyan S.E., Eremin I.I., Deev R.V. Experimental models of acute liver failure. Rossiyskiy mediko-biologicheskiy vestnik im. akademika I.P. Pavlova. 2015; (4): 138–44. (in Russian)
- Urazmetova M.D., Khadzhibaev F.A., Mirzakulov A.G., Akilov B.B. Experimental methods for the formation of acute liver failure (literature review and own research). Vestnik ekstrennoy meditsiny. 2018; 11(4): 66–72. (in Russian)
- Katikova O.Yu. The effect of mexidol on the state of homeostasis and lipid peroxidation in guinea pigs intoxicated with paracetamol. Eksperimentalnaya i klinicheskaya farmakologiya. 2002; 65(6): 53–6. (in Russian)
- Chernova V.M. Toxic liver damage: modern views and approaches to therapy. Ostrye i neotlozhnye sostoyaniya v praktike vracha. 2011; (2): 26–30. (in Russian)
- Konoplya A.I., Loktionov A.L., Dudka V.V., Dolgareva S.A., Sorokin A.V., Bushmina O.N. Chronic intoxication with ethanol: metabolic changes, correction of disturbances. Toksikologicheskiy vestnik. 2015; (5): 25–30. (in Russian)
- Myshkin V.A., Bakirov A.B. Oxymethyluracil. Essays on Experimental Pharmacology [Oksimetiluratsil. Ocherki eksperimental’noy farmakologii]. Ufa; 2001. (in Russian)
- Myshkin V.A., Bakirov A.B. Experimental Correction of Chemical Lesions of the Liver with Pyrimidine Derivatives (Efficacy and Mechanism of Action) [Eksperimental’naya korrektsiya khimicheskikh porazheniy pecheni proizvodnymi pirimidina (effektivnost’ i mekhanizm deystviya)]. Ufa; 2002. (in Russian)
- Myshkin V.A., Enikeev D.A. Correction of Post-Intoxication Disorders [Korrektsiya postintoksikatsionnykh narusheniy]. Ufa; 2005. (in Russian)
- Meleshin M.I., Skupnevskiy S.V, Chopikashvili L.V. Seasonal variations in hepatotoxicity of carbon tetrachloride in rats. Zdorove i obrazovanie v XXI Veke. Elektronnyy sbornik nauchnykh trudov. 2009; 11(10): 424–5. (in Russian)
- Council of Europe. European convention for the protection of vertebrate animals used for experimental and other scientific purpose. Strasbourg; 1986.
- Kamyshnikov V.S. Reference Book on Clinical and Biochemical Research and Laboratory Diagnostics [Spravochnik po kliniko-biokhimicheskim issledovaniyam i laboratornoy diagnostike]. Moscow: Medpress-inform; 2009. (in Russian)
- Mirsaev T.R. Hepatoprotective effect of oxymethyluracil. Farmatsiya. 2007; (2): 34–5. (in Russian)
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