Morphogenesis in vitro in calluses of lavender Lavandula angustifolia Mill.: histological aspects

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Histological events occurring in the calluses of Lavandula angustifolia Mill. at the initial stages of in vitro culture (1 passage) were described for the first time. It was found that the non-morphogenic callus is mainly represented by parenchymal tissue with few morphogenetic foci, mostly degenerated. In morphogenic calluses the morphogenesis pathways such as de novo organogenesis and indirect in vitro somatic embryogenesis have been identified and multiple developing morphogenetic foci have been noted also. The question of the realization of the pluri- and totipotency properties of callus cells in vitro is discussed. The histological data can be used in choosing the direction of application of regenerants obtained from calluses of this valuable essential oil and medicinal plant in various cell technologies.

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N. Kruglova

Research Institute of Agriculture of Crimea; Ufa Institute of Biology — Subdivision of the UFRC RAS

编辑信件的主要联系方式.
Email: kruglova@anrb.ru
俄罗斯联邦, Simferopol; Ufa

А. Zinatullina

Research Institute of Agriculture of Crimea; Ufa Institute of Biology — Subdivision of the UFRC RAS

Email: kruglova@anrb.ru
俄罗斯联邦, Simferopol; Ufa

N. Egorova

Research Institute of Agriculture of Crimea

Email: kruglova@anrb.ru
俄罗斯联邦, Simferopol

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2. Fig. 1. Primary non-morphogenic (a) and morphogenic (b) callus of L. angustifolia. Notation: LE, leaf explant; MC, morphogenic callus; NMC, non-morphogenic callus. Scale: 10 mm

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3. Fig. 2. Non-morphogenic callus of L. angustifolia of the 1st passage: a - general view; b - areas of parenchyma tissue (longitudinal section); c - established morphogenetic foci (transverse section); d - degenerated morphogenetic foci (transverse section). Symbols: DMO - degenerating morphogenetic centre, MND - embedded morphogenetic centre. Scale: a - 5 mm, b - 200 µm, c, d - 50 µm

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4. Fig. 3. Morphogenic callus of L. angustifolia 1st passage: general view (a); bud rudiment (b); formed bud apex (c, d); leaf primordia of the 1st order (e), bud (f); leaf (g). All sections are longitudinal. Symbols: AP - bud apex, ZP - bud rudiment, L - leaf, MC - morphogenic callus, P - bud, PL - leaf primordium of the 1st order. Scale: a - 5 mm, b, d - 50 µm, c, e - 100 µm, f, g - 200 µm

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5. Fig. 4. Somatic embryos of early embryogenesis stages in morphogenic callus of L. angustifolia of the 1st passage: globular (a, b), transitional to heart-shaped (c), heart-shaped (d) embryos. All sections are longitudinal. Symbols: MC - morphogenic callus, SG - somatic embryo. Scale: a-c - 100 µm, d - 200 µm

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6. Fig. 5. Morphogenetic foci in the thickness of morphogenic callus of the 1st passage of L. angustifolia: a, b - transverse sections, c, d - longitudinal sections. Symbols: ZMO - morphogenetic centre, MZP - meristematic zone of the bud, MC - morphogenic callus, SfMO - formed morphogenetic centre. Scale: a-c - 100 µm, d - 200 µm

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