INDEL-typing of strains of parahemolytic vibrions in vitro and in silico

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Abstract

The article presents the results of studying the distribution of Vibrio parahaemolyticus INDEL genotypes in the world and in Russia in vitro and in silico using the previously developed INDEL typing method based on the distribution of alleles of six loci (Vp967, Vp08, Vp619, Vp2256, VpA472, and Vp506). Among 2105 strains of V. parahaemolyticus taken in the study, 30 unique INDEL genotypes were identified, seven of which were identified as major. At the same time, geographical attachment between the genotype of parahaemolytic vibrions and the place of their isolation was revealed. The circulation of 17 INDEL genotypes was detected on the territory of the Russian Federation, the predominant of which was D2, found only in the Russian Federation. As a result, it was found that the proposed INDEL-typing method is a useful tool in conducting epidemiological investigations, which makes it possible to identify phylogenetic relationships between different isolates, which in turn makes it possible to draw conclusions about the sources of splinters of the pathogen to a specific territory.

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About the authors

Olga S. Chemisova

Don State Technical University

Email: chemisova@inbox.ru
ORCID iD: 0000-0002-4059-2878

Candidate of Sciences in Biology, Associate Professor

Russian Federation, Rostov-on-Don

Oksana A. Tsyrulina

The Federal Government Health Institution Rostov-on-Don Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights

Email: rykowskaya.oxana@yandex.ru
ORCID iD: 0000-0001-6176-2605

Candidate of Sciences in Biology, Senior Researcher

Russian Federation, Rostov-on-Don

Aleksey S. Vodopyanov

The Federal Government Health Institution Rostov-on-Don Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights

Email: alexvod@gmail.com
ORCID iD: 0000-0002-9056-3231

Candidate of Sciences in Medicine, Senior Researcher

Russian Federation, Rostov-on-Don

Sergey O. Vodopyanov

The Federal Government Health Institution Rostov-on-Don Plague Control Researsh Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights

Email: serge100v@gmail.com
ORCID iD: 0000-0003-4336-0439

Doctor of Sciences in Medicine, Leading Researcher

Russian Federation, Rostov-on-Don

Margarita M. Sagakyants

The Federal Government Health Institution Rostov-on-Don Plague Control Researsh Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights

Author for correspondence.
Email: margsagak@rambler.ru
ORCID iD: 0000-0001-8235-3288

Researcher

Russian Federation, Rostov-on-Don

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2. Dendrogram based on cluster analysis of INDEL loci allele distribution in V. parahaemolyticus strains

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