Platinum Polyoxoniobate: Stability, Cytotoxicity, and Cellular Uptake
- Authors: Yudkina A.V.1,2, Vokhtantsev I.P.1, Rychkov D.A.2,3, Volchek V.V.4, Abramov P.A.4, Sokolov M.N.4, Zharkov D.O.5
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Affiliations:
- SB RAS Institute of Chemical Biology and Fundamental Medicine
- Synchrotron Radiation Facility – Siberian Circular Photon Source SKIF, SB RAS Boreskov Institute of Catalysis
- SB RAS Institute of Solid State Chemistry and Mechanochemistry
- SB RAS Nikolaev Institute of Inorganic Chemistry
- Novosibirsk State University
- Issue: Vol 51, No 2 (2025)
- Pages: 362-371
- Section: Articles
- URL: https://archivog.com/0132-3423/article/view/682753
- DOI: https://doi.org/10.31857/S0132342325020141
- EDN: https://elibrary.ru/LBFJNY
- ID: 682753
Cite item
Abstract
Platinum polyoxometalates are Pt (IV) complexes containing bulky cluster ligands. We have shown previously that platinum polyoxoniobate [(Nb6O19)2{Pt(OH)2}2]12− (Pt-PON1) containing two Pt centers can covalently bind DNA. Here we have addressed the structural stability of Pt-PON1 and its conjugate with guanine at the N7 position, cytotoxicity of this compound, and its accumulation in living cells. Quantum mechanical modeling showed that the Pt-PON1 complex is unstable outside the crystal lattice, while its conjugate with guanine likely undergoes structural rearrangement quite easily. A decrease in the survival of Escherichia coli XL1-Blue and DH5α strains and human HEK293T and MCF-7 cell lines was observed already at 20 μM Pt-PON1 but at higher concentrations the compound was poorly soluble in biologically compatible media. Atomic emission spectroscopy for Pt and Nb showed that Pt-PON1 is efficiently taken up by human cells in a stoichiometry corresponding to the original complex. Thus, platinum polyoxometalates, provided their solubility can be improved, may be considered as promising antitumor agents.
Keywords
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About the authors
A. V. Yudkina
SB RAS Institute of Chemical Biology and Fundamental Medicine; Synchrotron Radiation Facility – Siberian Circular Photon Source SKIF, SB RAS Boreskov Institute of Catalysis
Author for correspondence.
Email: ayudkina@niboch.nsc.ru
Russian Federation, prosp. Lavrentieva 8, Novosibirsk 630090; Nikolskiy prosp. 1, Kol’tsovo, Novosibirsk Region 630559
I. P. Vokhtantsev
SB RAS Institute of Chemical Biology and Fundamental Medicine
Email: ayudkina@niboch.nsc.ru
Russian Federation, prosp. Lavrentieva 8, Novosibirsk 630090
D. A. Rychkov
Synchrotron Radiation Facility – Siberian Circular Photon Source SKIF, SB RAS Boreskov Institute of Catalysis; SB RAS Institute of Solid State Chemistry and Mechanochemistry
Email: ayudkina@niboch.nsc.ru
Russian Federation, Nikolskiy prosp. 1, Kol’tsovo, Novosibirsk Region 630559; ul. Kutateladze 18, Novosibirsk 630128
V. V. Volchek
SB RAS Nikolaev Institute of Inorganic Chemistry
Email: ayudkina@niboch.nsc.ru
Russian Federation, prosp. Lavrentieva 3, Novosibirsk 630090
P. A. Abramov
SB RAS Nikolaev Institute of Inorganic Chemistry
Email: ayudkina@niboch.nsc.ru
Russian Federation, prosp. Lavrentieva 3, Novosibirsk 630090
M. N. Sokolov
SB RAS Nikolaev Institute of Inorganic Chemistry
Email: ayudkina@niboch.nsc.ru
Russian Federation, prosp. Lavrentieva 3, Novosibirsk 630090
D. O. Zharkov
Novosibirsk State University
Email: ayudkina@niboch.nsc.ru
Russian Federation, ul. Pirogova 2, Novosibirsk 630090
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