Assessment of the role of TAAR9 gene knockout in impulsive and compulsive behavior in rats

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Аннотация

Trace amines and their receptors, in particular TAAR9, can act as potential regulators of the mechanisms of compulsivity and impulsivity, as addictive manifestations of behavior. The aim of this work was to evaluate the impulsive and compulsive components of the behavioral phenotype of rats with TAAR9 gene knockout. The assessment of impulsive behavioral elements was performed using the adapted Iowa Gambling Task (IGT). TAAR9-KO rats did not differ from wild-type rats in the IGT test and equally preferred the option associated with the greatest reward. The marble burying test was used to assess the compulsive component of behavior. The TAAR9-KO group demonstrated an increase in compulsive-like behavior compared to the control in the marble burying test. Thus, TAAR9-knockout rats demonstrate prerequisites for increased compulsive behavior, which is associated with the development of elements of gambling addiction.

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Авторлар туралы

D. Surov

Institute of Experimental Medicine

Хат алмасуға жауапты Автор.
Email: surovd01@mail.ru
Ресей, Saint Petersburg

A. Lebedev

Institute of Experimental Medicine

Email: surovd01@mail.ru
Ресей, Saint Petersburg

S. Pyurveev

Institute of Experimental Medicine; Saint Petersburg State Pediatric Medical University

Email: surovd01@mail.ru
Ресей, Saint Petersburg; Saint Petersburg

I. Zhukov

Institute of Experimental Medicine; Saint Petersburg State University

Email: surovd01@mail.ru
Ресей, Saint Petersburg; Saint Petersburg

K. Gramota

Institute of Experimental Medicine

Email: surovd01@mail.ru
Ресей, Saint Petersburg

D. Traktirov

Institute of Experimental Medicine

Email: surovd01@mail.ru
Ресей, Saint Petersburg

P. Shabanov

Institute of Experimental Medicine

Email: surovd01@mail.ru
Ресей, Saint Petersburg

Әдебиет тізімі

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2. Fig. 1. IGT test. 1 – starting chamber; 2 – arms with automatic feeders; 3 – microcontroller; 4 – personal computer; FR – reinforcement modes (the number of seeds that can be obtained with a certain probability).

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3. Fig. 2. Design of training of rats in the IGT test. P is the probability of receiving a reward within the reinforcement schedule; FR is the reinforcement schedule (the number of seeds that can be obtained with a certain probability).

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4. Fig. 3. Changes in the number of runs into the maze arms during the training and testing periods. 1 – training protocol, 2 – experimental protocol, 3 – testing after stabilization of the estrous cycle; *** p < 0.001. Rats of the WT and TAAR9-KO groups demonstrate a significant increase in runs according to the results of testing on day 21 compared to day 6 (repeated measurement two-way ANOVA; F (3.854, 53.95) = 59.03, p < 0.001). Data are presented as mean ± error of the mean (SEM).

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5. Fig. 4. IGT preference scores in WT and TAAR9-KO rats on the testing day. One-way ANOVA, F (2,17) = 1.894. Data are presented as mean ± SEM.

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6. Fig. 5. Number of buried marbles in WT and TAAR9-KO groups. Student's t-test: TAAR9-KO vs WT: * p < 0.05, ** p < 0.01. Data are presented as mean ± SEM.

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