Influence of ApoE gene polymorphism on development of obesity and blood biochemical parameters in coal mine workers

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Abstract

Introduction. The prevalence of obesity is becoming more relevant every year. Individuals with increased body weight show alterations in various biochemical parameters including lipid profile. One of the genes involved in the regulation of lipid metabolism is the APOE gene.
The aim of the work. To study the influence of ApoE gene on biochemistry parameters and BMI level in coal mine workers.
Materials and Methods. Data of one hundred thirty four coal mine workers were included. Venous blood was collected from all participants for DNA and serum isolation. Further genotyping was carried out for two polymorphic variants of ApoE gene (rs7412 and rs429358). Along with this, blood biochemical parameters were evaluated.
Results. While studying the effect of two polymorphic variants on BMI in subjects, a significant association was noted among carriers of E2/E3 and E3/E3 genotypes. Among biochemical indices, had an effect on cholesterol, folic acid, homocysteine, total calcium, low density lipoproteins, alkaline phosphatase,
γ-glutamyltransferase, and fibrinogen.
Limitations. The results obtained characterize the situation at a specific production site (coal mine) in a particular region (Russia, Western Siberia). In the future, the reproducibility of the results of our study will be tested on other, larger samples with a wide range of genetic variants related to lipid metabolism and eating behaviour.
Conclusion. The results obtained in this study may be useful for further work in the formation of certain risk groups and preventive measures.

About the authors

Anastasia A. Yakovleva

Kemerovo State University

Email: asya-kemerovo2012@yandex.ru
Engineer, Department of Genetics and Fundamental Medicine, Kemerovo State University, Kemerovo, 650000, Russian Federation

Varvara I. Minina

Kemerovo State University; Federal Research Center of Coal and Coal Chemistry, Siberian Branch of the Russian Academy of Sciences

Email: vminina@mail.ru
DSc (Biology), Head of the Department of Genetics and Fundamental Medicine, Kemerovo State University, Kemerovo, 650000, Russian Federation

Olga A. Soboleva

Federal Research Center of Coal and Coal Chemistry, Siberian Branch of the Russian Academy of Sciences

Email: soboleva.olga88@yandex.ru
leading processing engineer, Laboratory of Cytogenetics, Federal Research Center of Coal and Coal Chemistry, Siberian Branch of the Russian Academy of Sciences, Kemerovo, 650000, Russian Federation

Olga I. Borisova

Kemerovo State University

student of Kemerovo State University, Kemerovo, 650000, Russian Federation

Olga V. Bulatova

Kemerovo State University

Associate Professor, Department of Genetics and Fundamental Medicine, Kemerovo State University, Kemerovo, 650000, Russian Federation

Alexander Yu. Prosekov

Kemerovo State University

Email: aprosekov@rambler.ru
DSc (Engineering), Professor, Laboratory of Biocatalysis, Kemerovo State University, Kemerovo, 650000, Russian Federation

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