Distribution of polymorphic variants of the TNF-α and TNFRSF1α genes in miners with dust lung pathology
- Authors: Zhukova A.G.1,2, Kazitskaya A.S.1,2, Yadykina T.K.1, Logunova T.D.1
-
Affiliations:
- Research Institute for Complex Problems of Hygiene and Occupational Diseases
- Kuzbass Humanitarian and Pedagogical Institute of the FSBEI HE Kemerovo State University
- Issue: Vol 102, No 7 (2023)
- Pages: 670-674
- Section: OCCUPATIONAL HEALTH
- Published: 31.08.2023
- URL: https://archivog.com/0016-9900/article/view/638508
- DOI: https://doi.org/10.47470/0016-9900-2023-102-7-670-674
- EDN: https://elibrary.ru/blohxx
- ID: 638508
Cite item
Full Text
Abstract
Introduction. Long-term exposure to coal-rock dust on the body causes the development of systemic inflammation, one of the development mechanisms of which is an increase in the production of pro-inflammatory cytokines as follows: TNF-α, IL-1β, IL-2, IL-6. The development and course of occupational pathology were shown to depend on the individual characteristics of the body of those who works in hazardous conditions. In this regard, it is necessary to establish the significance of the polymorphism of the TNF-α and TNFRSF1α genes in the development of dust lung pathology in workers of the main occupations of the mines in the South of Kuzbass.
The aim of the study was to investigate the prevalence of polymorphic variants of the TNF-α (rs1800629) and TNFRSF1α (rs4149584) genes in miners with dust lung pathology.
Materials and methods. A survey of one hundred twenty seven miners working for a long time in the mines of the south of Kuzbass was carried out. Of these: 69 miners with prolonged exposure to high concentrations of coal-rock dust with a previously proven diagnosis of dust lung pathology, 58 miners without a proven diagnosis of dust lung pathology working in the same sanitary and hygienic conditions (control). The typing of the TNF-α (rs1800629) and TNFRSF1α (rs4149584) genes was carried out using the Real-Time method.
Results. The GG rs1800629 TNF-α genotype were shown to be a potent molecular genetic marker of the risk of developing dust lung pathology, the chance of detecting it in the group of the miners with dust pathology is almost 4 times higher than in the control group. The carriage of the A allele and the heterozygous AG rs1800629 TNF-α genotype reduce the probability of developing dust lung pathology. It has been established that the GG rs4149584 TNFRSF1α genotype can be a molecular and genetic marker of the risk of developing dust lung pathology, the chance of detecting it in the group of the miners with dust pathology is 2.6 times higher than in the control group.
Limitations. The study was limited by relatively small size of the groups under study.
Conclusion. The carriage of homozygous GG genotypes of the rs1800629 and rs4149584 polymorphic loci of the TNF-α and TNFRSF1α genes, respectively, is associated with the risk of developing dust lung pathology in miners in the South of Kuzbass. The carriage of the A allele and the heterozygous AG rs1800629 TNF-α genotype reduce the probability of developing dust lung pathology.
Compliance with ethical standards. The examination of the patients complied with the ethical standards of the Bioethical Committee of the Research Institute for Complex Problems of Hygiene and Occupational Diseases, elaborated in accordance with the Helsinki Declaration of the World Association “Ethical Principles for Conducting Scientific Medical Research Involving Humans” as amended in 2013 and “Rules of Clinical Practice in the Russian Federation”, approved by the Order of the Ministry of Health of the Russian Federation No. 266 of June 19, 2003. Each study participant gave informed voluntary written consent to participate in the study and to publish personal medical information in depersonalized form in the Hygiene and Sanitation, Russian journal.
Contribution:
Zhukova A.G. — the concept and design of the study, writing a text, editing;
Kazitskaya A.S. — collection and processing of material, statistical processing, editing;
Yadykina T.K. — collection and processing of material;
Logunova T.D. — editing.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: March 23, 2023 / Accepted: June 7, 2023 / Published: August 30, 2023
Keywords
About the authors
Anna G. Zhukova
Research Institute for Complex Problems of Hygiene and Occupational Diseases; Kuzbass Humanitarian and Pedagogical Institute of the FSBEI HE Kemerovo State University
Author for correspondence.
Email: nyura_g@mail.ru
ORCID iD: 0000-0002-4797-7842
MD, PhD, DSci., Associate Professor, head of the molecular-genetic and experimental studies laboratory, Research Institute for Complex Problems of Hygiene and Occupational Diseases, Novokuznetsk, 654041, Russian Federation; head of the natural sciences sub-department, Kuzbass Humanitarian and Pedagogical Institute of the Kemerovo State University, Novokuznetsk, 654041, Russian Federation.
E-mail: nyura_g@mail.ru
Russian FederationAnastasiya S. Kazitskaya
Research Institute for Complex Problems of Hygiene and Occupational Diseases; Kuzbass Humanitarian and Pedagogical Institute of the FSBEI HE Kemerovo State University
Email: noemail@neicon.ru
ORCID iD: 0000-0001-8292-4810
Russian Federation
Tatyana K. Yadykina
Research Institute for Complex Problems of Hygiene and Occupational Diseases
Email: noemail@neicon.ru
ORCID iD: 0000-0001-7008-1035
Russian Federation
Tatyana D. Logunova
Research Institute for Complex Problems of Hygiene and Occupational Diseases
Email: noemail@neicon.ru
ORCID iD: 0000-0003-1575-289X
Russian Federation
References
- Bukhtiyarov I.V., Golovkova N.P., Khelkovskiy-Sergeev N.A. Problems of health preservation in coal industry workers – new challenges and new solutions. Meditsina truda i promyshlennaya ekologiya. 2017; (12): 1–6. https://elibrary.ru/zxhfft (in Russian)
- Chebotarev A.G. Working environment and occupational morbidity of mine personnel. Gornaya promyshlennost’. 2018; (1): 92–5. https://doi.org/10.30686/1609-9192-2018-1-137-92-95 https://elibrary.ru/yvoshx (in Russian)
- Vanka K.S., Shukla S., Gomez H.M., James C., Palanisami T., Williams K., et al. Understanding the pathogenesis of occupational coal and silica dust-associated lung disease. Eur. Respir. Rev. 2022; 31(165): 210250. https://doi: 10.1183/16000617.0250-2021
- Mikhaylova N.N., Sazontova T.G., Alekhina D.A., Kazitskaya A.S., Zhdanova N.N., Prokop’ev Yu.A., et al. Features of intracellular protective mechanisms under the action of various xenobiotics on the body. Tsitokiny i vospalenie. 2013; 12(4): 71–5. https://elibrary.ru/shdxlh (in Russian)
- Panev N.I., Zakharenkov V.V., Korotenko O.Yu., Epifantseva N.N. Immune and cytokine mechanisms of the disorders of external respiration function in the miners with occupational dust pulmonary pathology. Meditsina truda i promyshlennaya ekologiya. 2015; 55(9): 109–10. https://elibrary.ru/umgrwr (in Russian)
- Kazitskaya A.S., Mikhaylova N.N., Zhukova A.G., Gorokhova L.G. Immune mechanisms underlying occupational bronchopulmonary diseases due to dust. Meditsina truda i promyshlennaya ekologiya. 2018; 58(6): 33–8. https://doi.org/10.31089/1026-9428-2018-6-33-38 https://elibrary.ru/xqmxbz (in Russian)
- Yucesoy B., Luster M.I. Genetic susceptibility in pneumoconiosis. Toxicol. Lett. 2007; 168(3): 249–54. https://doi.org/10.1016/j.toxlet.2006.10.021
- Yucesoy B., Johnson V.J., Kissling G.E., Fluharty K., Kashon M.L., Slaven J., et al. Genetic susceptibility to progressive massive fibrosis in coal miners. Eur. Respir. J. 2008; 31(6): 1177–82. https://doi.org/10.1183/09031936.00075107
- Maier L.A., Sawyer R.T., Bauer R.A., Kittle L.A., Lympany P., McGrath D., et al. High beryllium-stimulated TNF-alpha is associated with the -308 TNF-alpha promoter polymorphism and with clinical severity in chronic beryllium disease. Am. J. Respir. Crit. Care Med. 2001; 164(7): 1192–9. https://doi.org/10.1164/ajrccm.164.7.2012123
- Kim K.A., Cho Y.Y., Cho J.S., Yang K.H., Lee W.K., Lee K.H., et al. Tumor necrosis factor-alpha gene promoter polymorphism in coal workers’ pneumoconiosis. Mol. Cell Biochem. 2002; 234–235(1–2): 205–9. https://doi.org/10.1023/a:1015914409661
- Petsonk E.L., Rose C., Cohen R. Coal mine dust lung disease. New lessons from old exposure. Am. J. Respir. Crit. Care Med. 2013; 187(11): 1178–85. https://doi.org/10.1164/rccm.201301-0042CI
- Zhang M., Peng L.L., Ji X.L., Yang H.B., Zha R.S., Gui G.P. Tumor necrosis factor gene polymorphisms are associated with silicosis: a systemic review and meta-analysis. Biosci. Rep. 2019; 39(2): BSR20181896. https://doi.org/10.1042/BSR20181896
- Mukhammadieva G.F., Kutlina T.G., Karimov D.O., Bakirov A.B., Shagalina A.U., Idiyatullina E.F. Role of polymorphic variants of the genes TNFα, TSLP in the occupational asthma development. Ekologiya cheloveka. 2017; 24(10): 34–8. https://doi.org/10.33396/1728-0869-2017-10-34-38 (in Russian)
- Fomenko D.V., Ulanova E.V., Gromov K.G., Kazitskaya A.S., Bondarev O.I. Medical and biologic research of coal dust exposure as intoxication factor. Byulleten’ Vostochno-Sibirskogo nauchnogo tsentra Sibirskogo otdeleniya Rossiyskoy akademii meditsinskikh nauk. 2009; (1): 278–83. https://elibrary.ru/kysioz (in Russian)
- Kazitskaya A.S., Bondarev O.I., Bugaeva M.S., Zhukova A.G., Yadykina T.G. Morphometric and genetic studies of the mechanisms of damage to the cardiovascular system in Kuzbass miners with dust lung pathology. Meditsina truda i promyshlennaya ekologiya. 2021; 61(9): 611–9. https://doi.org/10.31089/1026-9428-2021-61-9-611-619 https://elibrary.ru/aqoooe (in Russian)
- Zhukova A.G., Kazitskaya A.S., Yadykina T.K., Korotenko O.Yu., Gulyaeva O.N. Association of HANP (RS5065) gene polymorphism with dust lung pathology and accompanying structural and functional changes in the myocardium among miners. Meditsina truda i promyshlennaya ekologiya. 2022; 62(5): 304–10. https://doi.org/10.31089/1026-9428-2022-62-5-304-310 https://elibrary.ru/itltws (in Russian)
- Sambrook J., Russell D.W. Purification of nucleic acids by extraction with phenol:chloroform. CSH Protoc. 2006; (1): pdb.prot4455. https://doi.org/10.1101/pdb.prot4455
- Bińkowski J., Miks S. Gene-Calc. Hardy-Weinberg equilibrium; 2018. Available at: https://gene-calc.pl/hardy-weinberg-page
- Holbrook J., Lara-Reyna S., Jarosz-Griffiths H., McDermott M. Tumour necrosis factor signalling in health and disease. F1000Res. 2019; 8: F1000 Faculty Rev-111. https://doi.org/10.12688/f1000research.17023.1
- Gaffney A., Christiani D.C. Gene-environment interaction from international cohorts: impact on development and evolution of occupational and environmental lung and airway disease. Semin. Respir. Crit. Care Med. 2015; 36(3): 347–57. https://doi.org/10.1055/s-0035-1549450
- Zhukova A.G., Kazitskaya A.S., Yadykina T.K., Panev N.I. Polymorphism of HIF-1A (RS11549465) and VEGFA (RS2010963) genes and the immune status in the dust lung pathology miners working at the coal enterprises in the south of Kuzbass. Gigiena i Sanitaria (Hygiene and Sanitation, Russian journal). 2021; 100(7): 683–7. https://doi.org/10.47470/0016-9900-2021-100-7-683-687 https://elibrary.ru/wsumfn (in Russian)
- Hamid T., Gu Y., Ortines R.V., Bhattacharya C., Wang G., Xuan Y.T., et al. Divergent tumor necrosis factor receptor-related remodeling responses in heart failure: role of nuclear factor-kappaB and inflammatory activation. Circulation. 2009; 119(10): 1386–97. https://doi.org/10.1161/CIRCULATIONAHA.108.802918
- Tarrats N., Moles A., Morales A., García-Ruiz C., Fernández-Checa J.C., Marí M. Critical role of tumor necrosis factor receptor 1, but not 2, in hepatic stellate cell proliferation, extracellular matrix remodeling, and liver fibrogenesis. Hepatology. 2011; 54(1): 319–27. https://doi.org/10.1002/hep.24388
- Qian Q.Z., Cao X.K., Liu H.Y., Zheng G.Y., Qian Q.Q., Shen F.H. TNFR/TNF-α signaling pathway regulates apoptosis of alveolar macrophages in coal workers’ pneumoconiosis. Oncotarget. 2017; 9(1): 1302–10. https://doi.org/10.18632/oncotarget.18921
- Bondarev O.I., Mal’tseva N.V., Surkov A.M., Bondareva I.A., Azarov P.A. Apoptotic activity as a trigger mechanism of systemic fibrosis of internal organs in miners. Meditsina v Kuzbasse. 2022; 21(3): 111–4. https://doi.org/10.24412/2687-0053-2022-3-111-114 https://elibrary.ru/nfedev (in Russian)
- Wilson A.G., Symons J.A., McDowell T.L., McDevitt H.O., Duff G.W. Effects of a polymorphism in the human tumor necrosis factor alpha promoter on transcriptional activation. Proc. Natl Acad. Sci. USA. 1997; 94(7): 3195–9. https://doi.org/10.1073/pnas.94.7.3195
- Elahi M.M., Asotra K., Matata B.M., Mastana S.S. Tumor necrosis factor alpha -308 gene locus promoter polymorphism: an analysis of association with health and disease. Biochim. Biophys. Acta. 2009; 1792(3): 163–72. https://doi.org/10.1016/j.bbadis.2009.01.007
- Dostert C., Grusdat M., Letellier E., Brenner D. The TNF Family of ligands and receptors: communication modules in the immune system and beyond. Physiol. Rev. 2019; 99(1): 115–60. https://doi.org/10.1152/physrev.00045.2017
- Zhang H., Sui J.N., Gao L., Guo J. Subcutaneous administration of infliximab-attenuated silica-induced lung fibrosis. Int. J. Occup. Med. Environ. Health. 2018; 31(4): 503–15. https://doi.org/10.13075/ijomeh.1896.01037
Supplementary files
