Research and comparative assessment of bioaccumulation and negative effects during chronic inhalation exposure to nano- and microparticles of aluminum oxide in an experiment

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

Introduction. In connection with atmospheric air pollution by Al2O3 nanoparticles (NPs), which have physical properties different from microparticles (MPs), it is relevant to identify the key features of the negative impact of Al2O3 NPs during long-term inhalation intake.

The aim of the study. Research and comparative assessment of bioaccumulation and negative effects associated with Al2O3 nano- and microparticles chronic inhalation intake the body in an experiment.

Materials and methods. The physical properties of Al2O3 NPs were studied in comparison with MPs. Using Wistar rats, the distinctive features of bioaccumulation and negative effects associated with chronic inhalation exposure to Al2O3 NPs at a concentration of 0.017 mg/m3 for 180 days were determined.

Results. Al2O3 NPs have a smaller size, larger specific surface area and total pore volume, which contributes to their greater penetrating activity through protective barriers compared to MPs. Due to this, with the same range of biodistribution organs, NPs have a higher degree of bioaccumulation. Exposure to NPs causes redox imbalance, cytolysis, hepatotoxic effect, disruption of the processes of excitation and inhibition of the nervous system, and thrombocytosis. When exposed to MP, less pronounced redox imbalance, cytolysis, and disruption of the process of neurotransmission of excitation were noted. NPs cause pathomorphological changes in the form of inflammation and impaired blood circulation in lung tissues, impaired blood circulation in the brain and liver. When exposed to MP, only the development of an inflammatory process in the lungs was established.

Limitations. The study was carried out only with chronic inhalation exposure to Al2O3 NPs and MPs on Wistar rats.

Conclusion. Al2O3 NPs have more pronounced bioaccumulation, a larger spectrum and degree of manifestation of negative effects in comparison with MPs. It is advisable to use the obtained results to improve the effectiveness of scientific substantiation of recommendations aimed at preventing and minimizing negative health effects associated with chronic inhalation exposure to Al2O3 NPs.

Compliance with ethical standards: the study was carried out in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental or other Scientific Purposes (ETS No. 123) and the requirements of the Ethics Committee of the Federal Scientific Center for Medical and Preventive Technologies for Public Health Risk Management (protocol No. 3 of 27.03.2018).

Contribution:
Zemlyanova M.A. — the concept and design of the study, processing of the material, editing, statistical processing;
Stepankov M.S. — collection of material, processing of material, writing the text.
All co-authors — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study was carried out at the expense of the federal budget.

Received: April 3, 2024 / Accepted: June 19, 2024 / Published: July 17, 2024

 

About the authors

Mark S. Stepankov

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Author for correspondence.
Email: stepankov@fcrisk.ru
ORCID iD: 0000-0002-7226-7682

MD, junior researcher, postgraduate student of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation

e-mail: stepankov@fcrisk.ru

Russian Federation

Marina A. Zemlyanova

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Perm State National Research University; Perm National Research Polytechnic University

Email: zem@fcrisk.ru
ORCID iD: 0000-0002-8013-9613

MD, PhD, DSci., assistant professor, head of Department of Biochemical and Cytogenetic Diagnostics of Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation; Perm State National Research University, Perm, 614990, Russian Federation; Perm National Research Polytechnic University, Perm, 614990, Russian Federation

e-mail: zem@fcrisk.ru

Russian Federation

References

  1. Maximize Market Research. Aluminium Oxide Nanoparticles Market – Global Industry Analysis and Forecast (2021–2027) by Application, End-User and Region. Available at: https://www.maximizemarketresearch.com/market-report/global-aluminium-oxide-nanoparticles-market/44118/
  2. Piracha S., Saleem S., Basharat G., Anjum A., Yaseen Z. Nanoparticle: role in chemical industries, potential sources and chemical catalysis applications. Sch. Int. J. Chem. Mater. Sci. 2021; 4(4): 40–5. https://doi.org/10.36348/sijcms.2021.v04i04.006
  3. Shafiq M., Anjum S., Hano C., Anjum I., Abbasi B.H. An overview of the applications of nanomaterials and nanodevices in the food industry. Foods. 2020; 9(2): 148. https://doi.org/10.3390/foods9020148
  4. Shafique M., Luo X. Nanotechnology in transportation vehicles: an overview of its applications, environmental, health and safety concerns. Materials (Basel). 2019; 12(15): 2493. https://doi.org/10.3390/ma12152493
  5. Salata O. Applications of nanoparticles in biology and medicine. J. Nanobiotechnology. 2004; 2(1): 3. https://doi.org/10.1186/1477-3155-2-3
  6. Liu X., Luo L., Ding Y., Xu Y. Amperometric biosensors based on alumina nanoparticles-chitosan-horseradish peroxidase nanobiocomposites for the determination of phenolic compounds. Analyst. 2011; 136(4): 696–701. https://doi.org/10.1039/c0an00752h
  7. Robertson T.A., Sanchez W.Y., Roberts M.S. Are commercially available nanoparticles safe when applied to the skin? J. Biomed. Nanotechnol. 2010; 6(5): 452–68. https://doi.org/10.1166/jbn.2010.1145
  8. Krewski D., Yokel R.A., Nieboer E., Borchelt D., Cohen J., Harry J., et al. Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide. J. Toxicol. Environ. Health B. Crit. Rev. 2007; 10(Suppl. 1): 1–269. https://doi.org/10.1080/10937400701597766
  9. Gupta R., Xie H. Nanoparticles in daily life: applications, toxicity and regulations. J. Environ. Pathol. Toxicol. Oncol. 2018; 37(3): 209–30. https://doi.org/10.1615/JEnvironPatholToxicolOncol.2018026009
  10. Boyuklieva R., Pilicheva B. Micro- and nanosized carriers for nose-to-brain drug delivery in neurodegenerative disorders. Biomedicines. 2022; 10(7): 1706. https://doi.org/10.3390/biomedicines10071706
  11. Yousef M.I., Roychoudhury S., Jafaar K.S., Slama P., Kesari K.K., Kamel M.A. Aluminum oxide and zinc oxide induced nanotoxicity in rat brain, heart, and lung. Physiol. Res. 2022; 71(5): 677–94. https://doi.org/10.33549/physiolres.934831
  12. Bhatti J.S., Bhatti G.K., Reddy P.H. Mitochondrial dysfunction and oxidative stress in metabolic disorders – A step towards mitochondria based therapeutic strategies. Biochim. Biophys. Acta Mol. Basis. Dis. 2017; 1863(5): 1066–77. https://doi.org/10.1016/j.bbadis.2016.11.010
  13. Bosco M., Kish T. Hepatotoxicity with elevated bilirubin secondary to prophylactic doses of unfractionated heparin: a case report and review of heparin-induced hepatotoxicity. J. Pharm. Technol. 2019; 35(1): 36–40. https://doi.org/10.1177/8755122518803363
  14. Al-Megrin W.A., Alkhuriji A.F., Yousef A.O.S., Metwally D.M., Habotta O.A., Kassab R.B., et al. Antagonistic efficacy of luteolin against lead acetate exposure-associated with hepatotoxicity is mediated via antioxidant, anti-inflammatory, and anti-apoptotic activities. Antioxidants (Basel). 2019; 9(1): 10. https://doi.org/10.3390/antiox9010010
  15. Alhusaini A., Fadda L., Hasan I.H., Zakaria E., Alenazi A.M., Mahmoud A.M. Curcumin ameliorates lead-induced hepatotoxicity by suppressing oxidative stress and inflammation, and modulating Akt/GSK-3β signaling pathway. Biomolecules. 2019; 9(11): 703. https://doi.org/10.3390/biom9110703
  16. Ilesanmi O.B., Adeogun E.F., Odewale T.T., Chikere B. Lead exposure-induced changes in hematology and biomarkers of hepatic injury: protective role of TrévoTM supplement. Environ. Anal. Health Toxicol. 2022; 37(2): e2022007-0. https://doi.org/10.5620/eaht.2022007
  17. Nazarenko G.I., Kishkun A.A. Clinical Evaluation of Laboratory Research Results [Klinicheskaya otsenka rezul’tatov laboratornykh issledovanii]. Moscow: Meditsina; 2006. (in Russian)
  18. Ahmed R.A. Hepatoprotective and antiapoptotic role of aged black garlic against hepatotoxicity induced by cyclophosphamide. J. Basic Appl. Zool. 2018; 79: 8. https://doi.org/10.1186/s41936-018-0017-7
  19. Modaberi S., Heysieattalab S., Shahbazi M., Naghdi N. Combination effects of forced mild exercise and GABAB receptor agonist on spatial learning, memory, and motor activity in striatum lesion rats. J. Mot. Behav. 2019; 51(4): 438–50. https://doi.org/10.1080/00222895.2018.1505711
  20. Kolasinski J., Hinson E.L., Divanbeighi Zand A.P., Rizov A., Emir U.E., Stagg C.J. The dynamics of cortical GABA in human motor learning. J. Physiol. 2019; 597(1): 271–82. https://doi.org/10.1113/JP276626
  21. Hsu Y.T., Chang Y.G., Chern Y. Insights into GABAAergic system alteration in Huntington’s disease. Open Biol. 2018; 8(12): 180165. https://doi.org/10.1098/rsob.180165
  22. Ambrad Giovannetti E., Fuhrmann M. Unsupervised excitation: GABAergic dysfunctions in Alzheimer’s disease. Brain Res. 2019; 1707: 216–26. https://doi.org/10.1016/j.brainres.2018.11.042
  23. Kaur R., Mehan S., Singh S. Understanding multifactorial architecture of Parkinson’s disease: pathophysiology to management. Neurol. Sci. 2019; 40(1): 13–23. https://doi.org/10.1007/s10072-018-3585-x
  24. Diana A., Pillai R., Bongioanni P., O’Keeffe A.G., Miller R.G., Moore D.H. Gamma aminobutyric acid (GABA) modulators for amyotrophic lateral sclerosis/motor neuron disease. Cochrane Database Syst. Rev. 2017; 1(1): CD006049. https://doi.org/10.1002/14651858.CD006049.pub2
  25. Dicks O.A., Cottom J., Shluger A.L., Afanas’ev V.V. The origin of negative charging in amorphous Al2O3 films: the role of native defects. Nanotechnology. 2019; 30(20): 205201. https://doi.org/10.1088/1361-6528/ab0450
  26. Manuja A., Kumar B., Kumar R., Chhabra D., Ghosh M., Manuja M., et al. Metal/metal oxide nanoparticles: Toxicity concerns associated with their physical state and remediation for biomedical applications. Toxicol. Rep. 2021; 8: 1970–8. https://doi.org/10.1016/j.toxrep.2021.11.020
  27. Kaptein F.H.J., Kroft L.J.M., Hammerschlag G., Ninaber M.K., Bauer M.P., Huisman M.V., et al. Pulmonary infarction in acute pulmonary embolism. Thromb. Res. 2021; 202: 162–9. https://doi.org/10.1016/j.thromres.2021.03.022

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Stepankov M.S., Zemlyanova M.A.


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 37884 от 02.10.2009.