Evaluation of the potentiating effect of polystyrene microparticles on the toxicity of acrylamide and ethanol under conditions of combined treatment of mouse hepatocyte cell culture MH-22a

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Introduction. Microparticles of polymer compounds are common in the environment. polystyrene particles are the most common types of microplastics. The most interesting subject of the study is the assessment of the potentiating properties of microplastics on the manifestations of toxicity of common substances entering the body by alimentary means, primarily such as acrylamide and ethanol.

Materials and methods. The experimental work was performed on a cell culture of mouse MH-22a hepatocytes in compliance with the principles of working with mammalian cell cultures. An MTT test was used to study cell viability by respiratory activity. The statistical analysis was performed in the SPSS Statistics 21 software.

Results. The article presents the results of an experimental study of the respiratory activity of cells under combined treatment with 300 nm polystyrene microparticles at a concentration of 0.025% with acrylamide and ethanol. Preliminary experimental data is presented to substantiate the selected concentration of the microplastic under study, demonstrating its low acute cytotoxicity. The calculated IC50 values for cell survival for acrylamide and ethanol under single exposure and combined exposure with polystyrene microparticles for 24 hours had insignificant differences.

Limitations. The study was performed on a cell culture of mouse MH-22a hepatocytes (monolayer) cultured in accordance with the requirements of the culture passport and treated with 300 nm polystyrene microparticles and their mixtures with acrylamide and ethanol only for 24 hours in microplate format.

Conclusion. A comparative analysis of survival values when exposed to toxic substances without addition and in the presence of microplastics revealed no significant differences between cell groups, which at the moment did not allow detecting the potentiating effect of polystyrene microparticles with a size of 300 nm on the toxicity of acrylamide and ethanol under 24-hour combined treatment.

Compliance with ethical standards. The study was carried out in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (ETS No. 123), Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes and the requirements of the Bioethical Commission of the Ufa Research Institute of Occupational Health and Human Ecology” (protocol No. 01-02 of 08.02.2024).

Contribution:
Kudoyarov E.R. – writing text, collection and processing of material;
Karimov D.O. – concept and design of the study;
Gizatullina A.A. – collection and processing of material;
Karimov D.D. – editing;
Baygildin S.S. – collection and processing of material;
Yakupova T.G. – collection and processing of material.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgements. The study had no sponsorship.

Received: July 27, 2024 / Revised: May 25, 2024 / Accepted: June 19, 2024 / Published: July 31, 2024

作者简介

Eldar Kudoyarov

Ufa Research Institute of Occupational Health and Human Ecology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

编辑信件的主要联系方式.
Email: e.kudoyarov@yandex.ru
ORCID iD: 0000-0002-2092-1021

Junior researcher, Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation

e-mail: e.kudoyarov@yandex.ru

俄罗斯联邦

Denis Karimov

Ufa Research Institute of Occupational Health and Human Ecology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: noemail@neicon.ru
ORCID iD: 0000-0003-0039-6757

MD, PhD, head of the Dept. of toxicology and genetics with an experimental laboratory animal clinic Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation

俄罗斯联邦

Alina Gizatullina

Ufa Research Institute of Occupational Health and Human Ecology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: noemail@neicon.ru
ORCID iD: 0000-0002-7321-0864

Junior researcher, Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Health and Human, Ufa, 450106, Russian Federation

俄罗斯联邦

Denis Karimov

Ufa Research Institute of Occupational Health and Human Ecology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: noemail@neicon.ru
ORCID iD: 0000-0002-1962-2323

MD, PhD, senior researcher, Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation

俄罗斯联邦

Samat Baygildin

Ufa Research Institute of Occupational Health and Human Ecology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: noemail@neicon.ru
ORCID iD: 0000-0002-1856-3173

MD, PhD, researcher, Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation

俄罗斯联邦

Tatyana Yakupova

Ufa Research Institute of Occupational Health and Human Ecology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: noemail@neicon.ru
ORCID iD: 0000-0002-1236-8246

Junior researcher, Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation

俄罗斯联邦

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