V.F.Snegirev Archives of Obstetrics and GynecologyV.F.Snegirev Archives of Obstetrics and Gynecology2313-87262687-1386Eco-Vector35262Research ArticleVaginal microbiocenosis in patients with HPV-associated and HPV-negative cervical intraepithelial neoplasiasKononovaI. N-VoroshilinaE. Svoroshilina@gmail.comZornikovD. L-MalyginA. G-Ural State Medical UniversityHarmony Center1503201521222621072020Copyright © 2015, Eco-Vector2015Vaginal microbiocenosis was studied in 311 patients with cervical intraepithelial neoplasias (CIN) by RT-PCR (Femoflor-16 test, DNA-Technologies). The results indicated that HPV-asociated and HPV-negative CIN were associated with the development of pronounced vaginal dysbiosis caused by mainly obligate anaerobes, predominantly Gardnerella vaginalis in associations with Atopobium vaginae, Megasphaera spp./Veillonella spp./Dialister spp., and Eubacterium spp. A great variety of the agents dictated comprehensive studies of the vaginal microbiocenosis in patients with cervical precancer conditions in order to prescribe individual therapy.LSILHSILvaginal microbiocenosisanaerobic dysbiosiscervical intraepithelial neoplasiasLSILHSILмикробиоценоз влагалищаанаэробный дисбиозцервикальные интраэпителиальные неоплазии[Долгушина В.Ф., Колесников О.Л., Эрлихман Э.Н. Микробиоценоз влагалища у женщин с хроническим цервицитом, ассоциированным с герпетической инфекцией. Вестник новых медицинских технологий. 2008; 40 (3): 165-6.][Патология шейки матки и генитальные инфекции / Под ред. В.Н. Прилепской. М.: МЕДпресс-информ; 2008.][Gillet E., Meys J.F., Verstraelen H., Bosire C., De Sutter P., Temmerman M. et al. Bacterial vaginosis is associated with uterine cervical human papillomavirus infection: a meta-analysis. BMC Infect. Dis. 2011; 11 (10): 1287-95.][Watts D.H., Fazzari M., Minkoff H., Hillier S.L., Sha B., Glesby M. et al. Effects of bacterial vaginosis and other genital infections on the natural history of human papillomavirus infection in HIV-1-infected and high-risk HIV-1-uninfected women. J. Infect. Dis. 2005; 191 (7): 1129-39.][Clarke M.A., Rodriguez A.C., Gage J.C., Herrero R., Hildesheim A., Wacholder S. A large, population-based study of age-related associations between vaginal pH and human papillomavirus infection. BMC Infect. Dis. 2012; 12 (33): 1286-92.][Peters N., Van Leeuwen A.M., Pieters W.J., Hollema H., Quint W.G., Burger M.P. Bacterial vaginosis is not important in the etiology of cervical neoplasia: a survey on women with dyskaryotic smears. Sex. Transm. Dis. 1995; 22 (5): 296-302.][Boyle D.C., Barton S.E., Uthayakumar S., Hay P.E., Pollock J.W., Steer P.J. et al. Is bacterial vaginosis associated with cervical intraepithelial neoplasia? Int. J. Gynecol. Cancer. 2003; 13 (2): 159-63.][Nam K.H., Kim Y.T., Kim S.R., Kim S.W., Kim J.W., Lee M.K. et al. Association between bacterial vaginosis and cervical intraepithelial neoplasia. J. Gynecol. Oncol. 2009; 20 (1): 39-43.][Klomp J.M., Boon M.E., Van Haaften M. et al. Cytologically diagnosed Gardnerella vaginalis infection and cervical (pre)neoplasia as established in population-based cervical screening. Am. J. Obstet. Gynecol. 2008; 199: 480-5.][Atashili J., Poole C., Ndumbe P.M., Adimora A.A., Smith J.S. Bacterial vaginosis and HIV acquisition: a meta-analysis of published studies. AIDS. 2008; 22: 1493-501.][Кира Е.Ф., Душкина Е.А., Бадикова Н.С. Биологическая роль кислотности влагалища. Механизмы стабильности и методы коррекции. Акушерство и гинекология. 2013; 3: 102-6.][Кира Е.Ф. Бактериальный вагиноз. М.: МИА; 2012.][Роговская С.И. Папилломавирусная инфекция у женщин и патология шейки матки. 2-e изд. М.: ГЭОТАР-Медиа; 2011.][Ворошилина Е.С. Совершенствование методических подходов к оценке микробиоценоза влагалища у женщин репродуктивного возраста. Дисс.. д-ра мед. наук. Челябинск: Уральская государственная медицинская академия; 2012.][Mitra A., Yun L., Macintyre D. et al. Assessment of the vaginal microbiome with bacterial DNA pyrosequencing assording to CIN grade and severity. In: Abstracts of the 15 World Congress for Cervical Pathology and Colposcopy. 2014: 123-4.]