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Fig. 3. Expression of the TurboGFP-dest1 reporter protein under the control of the FOS or RPLP0 promoter in HEK 293T cells. a – Confocal micrographs of HEK 293T cells transfected with pFOS-TurboGFP or pRPLP0-TurboGFP. Forty-eight hours after transfection, the cells were incubated in the presence of 1 μM ouabain for 3 h, in Na or Li medium for 5 h. Scale bar: 100 μm. b – Distributions of the mean fluorescence intensity of TurboGFP-dest1 in individual cells (n = 500–1000). Statistical analysis of the data revealed a small but significant increase in reporter protein fluorescence in response to ouabain compared to the control (DMEM). A similar effect was observed in cells incubated in Li-medium compared to Na-medium. Statistically significant differences were identified using the Mann–Whitney U-test (* p < 0.05) |