Europium Complexes with Fluorinated Carbazole-Containing Tetraketones

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Resumo

Complex compounds of fluorine-containing tetraketones on the basis of carbazole with europium(III) ions were synthesized, the luminescence-spectral properties of the obtained complexes were evaluated and the exact stoichiometric composition was established. The position of the maximum in the excitation spectrum (≥ 370 nm) of the complexes, a significant Stokes shift (> 250 nm), and a long lifetime of the excited state (400–700 μs) allow us to consider the synthesized complexes as potential reagents for time-resolved fluorescence immunoassay.

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Sobre autores

Anna Shubina

State University of Education

Autor responsável pela correspondência
Email: shubina.anna98@yandex.ru
ORCID ID: 0009-0004-7466-5037
Rússia, Moscow

Tatiana Kostryukova

State Research Institute of Biological Instrumentation

Email: shubina.anna98@yandex.ru
Rússia, Moscow

Dmitry Pugachev

State Research Institute of Biological Instrumentation

Email: shubina.anna98@yandex.ru
ORCID ID: 0000-0002-2042-3911
Rússia, Moscow

Dmitry Paramonov

State Research Institute of Biological Instrumentation

Email: shubina.anna98@yandex.ru
ORCID ID: 0009-0009-8005-8504
Rússia, Moscow

Sergey Vatsadze

Zelinsky Institute of Organic Chemistry

Email: shubina.anna98@yandex.ru
ORCID ID: 0000-0001-7884-8579
Rússia, Moscow

Nikolay Vasilyev

State University of Education; State Research Institute of Biological Instrumentation

Email: shubina.anna98@yandex.ru
ORCID ID: 0000-0003-4146-3979
Rússia, Moscow; Moscow

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2. Scheme 1.

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3. Fig. 1. Dependence of luminescence intensity on the Eu3+-ligand ratio.

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4. Scheme 2.

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5. Scheme 3.

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6. Scheme 4.

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7. Fig. 2. Typical 1H NMR spectrum of the example of the complex with methyl substituted ligand 2.

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8. Fig. 3. Fragments of IR spectra of ligands 1-4 (a, c, e, g) and their complexes 6-9 (b, d, e, h).

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9. Fig. 4. Absorption spectra of the original ligands 1-4 and the widely used in immunofluorescence analysis naphthoyltrifluoroacetone (NTA) [2].

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10. Fig. 5. Absorption spectra of complexes 6-9 compared to the NTA-based complex.

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11. Fig. 6. Comparison of the luminescence spectra of complexes 6-9 with the NTA-based complex.

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